Repository landing page
Single-channel recordings with HlyA<sub>Δ158–167</sub> and HlyA<sub>Δ180–203</sub>.
Abstract
<p>Single-channel recordings of asolectin membranes were performed in the presence of 100 ng/ml HlyA<sub>Δ158–167</sub> (upper trace) and 150 ng/ml HlyA<sub>Δ180–203</sub> (lower trace). The aqueous phase contained 150 mM KCl (pH 6). The applied membrane potential was 20 mV; T = 20°C. The transient conductance steps in the upper trace (HlyA<sub>Δ158–167</sub>) had a conductance of about 600 to 700 pS. The mutant HlyA<sub>Δ180–203</sub> produced under the given conditions only current noise (fuzzy channels) and no defined conductance states.</p- Image
- Figure
- Biological Sciences
- 110 kDa
- Several Amino
- pore formation
- channel structure
- structure predictions
- channel diameters
- acid residues 238
- lipid bilayers
- RTX toxins
- Osmotic protection experiments
- Lipid bilayer measurements
- mutants part
- HlyA transmembrane channel
- wildtype HlyA channels
- wildtype toxin
- 1.8 nm
- HlyA mutants
- wildtype HlyA
- transmembrane pores