Double immunofluoresence staining of CD90 (<i>green</i>) and αSMA (<i>red</i>) on PDAC tissues.

Abstract

<p>DAPI counterstaining was used to visualize nuclei (<i>blue</i>). <i>Yellow</i> or <i>orange</i> represent the co-localization of CD90 and αSMA. (A) A stage I PDAC specimen exhibited a single layer of CD90⁺ activated PSCs (<i>yellow</i>) surrounding the duct-like structure. (B) Higher magnification of the stromal area in PDAC displaying an apparent overlap between CD90 and αSMA (y<i>ellow</i> or <i>orange</i> in the merged images). The IHC result showed that CD90 mostly overlapped with αSMA in PDAC stroma, demonstrating that CD90⁺ stromal cells consist largely of activated PSCs.</p