<i>CCR5</i> gene disruption by RNA-guided Cas9 endonuclease in sorted CCR5-/CR1, CCR5-/CR2 and CCR5-/CR3 cells by Sanger sequencing analysis.

Abstract

<p>A-C. Cell surface expression of CCR5 on sorted CCR5-/CR1 (A), CCR5-/CR2 (B) and CCR5-/CR3 (C) TZM.bl cells as compared to mock-transduced TZM.bl cells. D-F. Representative Sanger sequencing of CCR5 target sites by CR1 (D), CR2 (E) or CR3 (F) in co-transduced TZM.bl cells. The full list of Sanger sequencing of CCR5 target sites by CR1, CR2 or CR3 is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115987#pone.0115987.s002" target="_blank">S2 Fig</a>. For each <i>CCR5</i> site targeted by CR1 (D), CR2 (E) or CR3 (F), the upper panel shows representative Sanger sequencing of targeted CCR5 sequences amplified by a prime pair that covers both the endogeous <i>CCR5</i> gene and transgene. The lower panel shows representative Sanger sequencing of targeted <i>CCR5</i> sequences by a prime pair that only covers the endogenous <i>CCR5</i> gene.</p