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Protein expression levels of WT- and Y219*-AQP0 transfected into HEK 293T cells.
Abstract
<p>Western blots were performed with the anti-FLAG as indicated.β-actin was used as the loading control. Cells with the mutated (Y219*) AQP0 construct showed an 87% reduction in AQP0 protein level compared to cells with wild-type AQP0. ***P<0.01</p- Image
- Figure
- Biological Sciences
- mip
- Chinese family
- Novel Nonsense Mutation
- cytoplasm.ConclusionsThe novel nonsense mutation
- protein expression levels
- Chinese Family PurposeTo
- restriction fragment length polymorphism
- cataract
- cell membrane
- HEK 293 T cells
- dna
- HEK 293T cells
- Congenital Posterior Polar Cataracts
- aqp
- rflp