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<i>Myb and Cebpa</i> knockdown in primary KIT<sup>+</sup> enriched bone marrow cells.
Abstract
<p>(A) Two-dimensional flow cytometry dot plot showing the analysis of the KSL compartment of a KIT<sup>+</sup> enriched population transfected with siRNA control or undergoing siRNA-mediated silencing of <i>Myb</i> or <i>Cebpa</i> for 20 hours. (B) Grouping four independent experiments, boxplots depict the variations in percentage of FLT3<sup>+</sup> cells within the KSL populations. (C) Quantitative PCR analysis of <i>Myb</i> and <i>Cebpa</i> RNA expression in sorted transfected KSL cells 20 hours post transfection. Expression is normalised to <i>β</i><sub><i>2</i></sub><i>-microglobulin</i> and standardised to the control samples. Error bars represent the standard error of the mean. Plots are representative of 4 independent experiments. Numbers are plotted as mean ± SEM (**, p<0.001 and *, p<0.05).</p- Image
- Figure
- Uncategorised
- influence lineage commitment
- MYB transcription factor
- Flt 3 Gene
- Haematopoietic Stem
- progenitor compartment
- intronic elements
- Myb gene
- proliferation capacity
- hsc
- myeloid leukaemia
- Flt 3 promoter
- Flt 3 expression
- chromatin level
- multipotential progenitors
- Flt 3 transcriptional regulation
- myeloid commitment
- progenitor cells
- MYB activity
- cell context
- vivo deletion
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Last time updated on 12/02/2018
This paper was published in The Francis Crick Institute.
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