The Tie1 T794 mutant disrupts endothelial tube formation.

Abstract

<p><b>a</b> Representative images of HUVECs left uninfected or infected with adenoviruses encoding GFP, Tie1-WT, or Tie1-T794A, plated on Matrigel and allowed to form tubes for 6 hours. <b>b, c</b> Quantification of endothelial tube networks <i>in vitro</i>. Cells expressing the T794A mutant formed significantly shorter tubes (<b>b</b>; *, multiple comparisons P≤ 0.02) with fewer nodes and branches (<b>c</b>; *, multiple comparisons P< 0.01) (n = 11 independent experiments with 4 or more images per experiment).</p