Repository landing page

We are not able to resolve this OAI Identifier to the repository landing page. If you are the repository manager for this record, please head to the Dashboard and adjust the settings.


<p>(A) mRNA-Seq expression values for 25/368 of the mRNAs identified by Venn analysis (<a href="" target="_blank">Fig 3C</a>; right panel, underlined) that are reduced by <i>Ire1α</i> because of glucose that accumulates in the <i>KO</i><sup><i>Fe/-; Cre</i></sup> ([<i>n</i> = 5], [<i>p</i>-values ≤ 0.05]). (B) Oxidized lipid (hydroxyl-octadecadienoic acids, HODEs) from islets of the indicated genotypes infected with <i>Ad-Cre Ad-GFP</i> or no virus control ([<i>n</i> = 2; controls versus <i>n</i> = 3; <i>Ad-Cre</i>], [<i>p</i> = 0.00434]). (C) Antinitrotyrosine immunohistochemistry (IHC) of islets from 8-mo-old <i>WT</i><sup><i>Fe/Fe</i></sup> and <i>KO</i><sup><i>Fe/Fe; Cre</i></sup> mice 15 wk post-Tam with or without BHA diet for 3 wk. (Scale bar, 50 μm) (<i>WT</i><sup><i>Fe/Fe</i></sup> [<i>n</i> = 4 with BHA], [<i>n</i> = 5 regular chow]), (<i>KO</i><sup><i>Fe/Fe; Cre</i></sup> [<i>n</i> = 5 with BHA], [<i>n</i> = 6 regular chow]). (<i>p</i> = 0.00698; <i>WT</i><sup><i>Fe/Fe</i></sup> versus <i>WT</i><sup><i>Fe/Fe</i></sup> with BHA), (<i>p</i> = 0.04018; <i>WT</i><sup><i>Fe/Fe</i></sup> versus <i>KO</i><sup><i>Fe/Fe; Cre</i></sup>) and (<i>p</i> = 0.04420; <i>KO</i><sup><i>Fe/Fe; Cre</i></sup> versus <i>KO</i><sup><i>Fe/Fe; Cre</i></sup> with BHA). (D) Masson’s trichrome stain (blue) for collagens. Results demonstrate increased staining surrounding <i>KO</i><sup><i>Fe/Fe; Cre</i></sup> islets with haemotoxylin (red) and eosin (black) co-stains. Quantification of percent strong collagen stain is shown below the images. Scale bar, 50 μm. (<i>WT</i><sup><i>Fe/Fe</i></sup> [<i>n</i> = 4 with BHA], [<i>n</i> = 5 regular chow]), (<i>KO</i><sup><i>Fe/Fe; Cre</i></sup> [<i>n</i> = 5 with BHA], [<i>n</i> = 6 without BHA]). Percent strong collagen stain significance for <i>WT</i><sup><i>Fe/Fe</i></sup> without BHA versus <i>KO</i><sup><i>Fe/Fe; Cre</i></sup> without BHA <i>p</i> = 0.01049). (E) 8-mo-old male mice carrying the doubly floxed allele (<i>Ire1α</i><sup><i>Fe/Fe</i></sup><i>)</i> with and without RIP-Cre 12 wk post-Tam had their pre-BHA GTTs taken, and then half were fed the antioxidant BHA supplemented chow diet for 3 wk or not before examining the mice by GTT again. (<i>WT</i><sup><i>Fe/Fe</i></sup> [<i>n</i> = 11 with BHA], [<i>n</i> = 12 regular chow], [<i>p</i> = 0.035]), (<i>KO</i><sup><i>Fe/Fe; Cre</i></sup> [<i>n</i> = 18 with BHA], [<i>n</i> = 16 without BHA], [<i>p</i> = 0.041]). <i>P</i>-values were calculated by one-tailed student’s <i>t</i> test comparison of the areas under the GTT curves for the biological replicates of control group <i>WT</i><sup><i>Fe/Fe</i></sup> versus the Tam-induced <i>KO</i><sup><i>Fe/Fe; Cre</i></sup> group.</p

Similar works

Full text



Last time updated on 12/02/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.