Estimations of the ability of antibodies or aptamers to recognized their epitopes.

Abstract

<p><b>(A)</b> Scheme explaining how the epitope recognition estimation was calculated. (I) Cellular intensity (% of aptamer) is the data also displayed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173050#pone.0173050.g003" target="_blank">Fig 3B</a>. (II) Intensity of single antibody package expressed as fold over the aptamer intensity is the data on <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173050#pone.0173050.g004" target="_blank">Fig 4A–4C</a>. (III) If we know the signal contribution of a single antibody package to the total cellular staining, we can estimate how many epitopes were found by the antibodies in relation to the epitopes found by the aptamers. (<b>B</b>) The results of the equation (III). Error bars represent the SEM from at least 3 independent experiments.</p