Exercise increased DJ-1, Hsp70 and BDNF levels in 15-month old Y39C transgenic mouse brain and also increased DJ-1 levels in muscle and plasma.

Abstract

<p>(<b>A</b>) Brain tissues (cortex) were processed for Western blot analysis using antibodies to DJ-1, Hsp70, BDNF and β-actin after 3 months of running wheel Exercise (<b>Ex</b>) or no Exercise (<b>nEx</b>) in Y39C transgenic animals. Representative images are shown for all Western blots. (<b>B-D</b>) Quantitative protein levels in brain are shown for each group after being normalized to β-actin. There were significant increases in DJ-1, Hsp70 and BDNF proteins in Exercise mouse brain compared to Non-Exercise mice (n = 7, t-test, *<i>p</i><0.05, **<i>p</i><0.01). (<b>E)</b> Muscle samples were processed for Western blot analysis using DJ-1 and β-actin antibodies after 3 months of running wheel Exercise (<b>Ex</b>) or no Exercise (<b>nEx</b>) in control animals. (<b>F</b>) Muscle DJ-1 Westerns were quantified and are shown for each group after being normalized to β-actin. Muscle DJ-1 was significantly increased in Exercise mice compared to Non-Exercise mice (n = 7, t-test, **<i>p</i><0.01). (<b>G</b>) Plasma DJ-1 levels were measured by ELISA. Results show that the Exercise group had significantly higher plasma DJ-1 concentrations than the Non-Exercise mice (n = 7, t-test, **<i>p</i><0.01).</p