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Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis

By Juergen von Zum Hof, Karsten Schonrogge, James M. Cook and Michael G. Gardner

Abstract

To date there have been only limited fine-scale investigations into the molecular ecology of the European\ud hoverfly, Microdon mutabilis, due to the paucity of available polymorphic markers.Wedescribe the development of primers amplifying five novel microsatellite loci using next-generation sequencing (454) and three previously undescribed M. mutabilis microsatellite loci using enrichments. In hoverflies from a population in Ireland, the number of alleles per locus ranged from 2 to 16, and the observed heterozygosity ranged between 0.26 and 0.97

Topics: Ecology and Environment
Year: 2012
DOI identifier: 10.1071/ZO12042
OAI identifier: oai:nora.nerc.ac.uk:19884

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