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Ultrasound-Guided Intrauterine Labeling of Rat Fetuses

By Hatice Gulcin Gumus, Miriam Illa, Laura Pla, Anna Gonzalez-Neira, Elena Demicheva, David Ley, Fatima Crispi and Eduard Gratacos


Aim: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. Methods: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared. Results: Survival rates (84.4% for intra-amniotic injections vs. 90.9% for fetal subcutaneous injections) and injection success rates (94% for intra-amniotic injections vs. 100% for fetal subcutaneous injections) were similar among both groups. None of the neonates from the intra-amniotic injections group were labeled at birth, while 93% of the neonates from fetal subcutaneous injections group were tagged, showing a visible spot in the skin at birth. Conclusion: Our results suggest that ultrasound-guided fetal subcutaneous injections might be an adequate strategy for selectively labeling fetuses in multifetal pregnant animals

Topics: Other Basic Medicine, Animal model, Fetal subcutaneous tagging, Intra-amniotic injections, Pregnancy, Ultrasound-guided injections, Vital dyes
Publisher: 'S. Karger AG'
Year: 2017
DOI identifier: 10.1159/000454766
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