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Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion

By Véronique Proux-Gillardeaux, Julie Gavard, Theano Irinopoulou, René-Marc Mège and Thierry Galli

Abstract

A role for endocytosis and exocytosis in cell migration has been proposed but not yet demonstrated. Here we show that cellubrevin, an early endosomal v-SNARE, mediates trafficking in the lamellipod of migrating epithelial cells and partially colocalizes with markers of focal contacts. Expression of tetanus neurotoxin, which selectively cleaves cellubrevin, significantly reduced the speed of migrating epithelial cells. Furthermore, expression of tetanus neurotoxin enhanced the adhesion of epithelial cells to collagen, laminin, fibronectin and E-cadherin, altered spreading on collagen, and impaired the recycling of ?1 integrins. These results suggest that cellubrevin-dependent membrane trafficking participates in cell motility through the regulation of cell adhesion

Topics: membrane traffic, clostridial neurotoxin, cell adhesion, cell migration, exocytosis, endocytosis, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Publisher: National Academy of Sciences
Year: 2005
DOI identifier: 10.1073/pnas.0409613102
OAI identifier: oai:HAL:hal-00007763v1
Provided by: Hal-Diderot
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