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Intracellular multiplex detection and imaging of stable chemisorbed labels by SERS spectroscopy

By N.M.S. Sirimuthu, C.D. Syme and J.M. Cooper


SERS spectroscopy is currently gaining wider acceptance in biological research due to its ability to obtain signals from very low quantities of material, and to obtain information from within live cells. SERS spectroscopy yields very narrow bands (10-100 times narrower than typical fluorescence bands) and spectra suffer from minimal interference from aqueous media, making SERS spectroscopy ideal for multiplex detection of intracellular components. Typically for sensing, nanoparticles are labelled with suitable sensing molecules such as a dye or thiol. Nanoparticle labelling involves two different types of interaction between the label and the enhancing surface, chemisorption and physisorption. The former is considerably stronger and more stable than the latter and hence chemisorbed labels are more appropriate for intracellular nanosensor design. In this paper, we demonstrate the difference in stability of both types of Raman label inside live cells over periods of time. Chinese hamster ovary (CHO) cells were infused with a mixture of differently labelled stable nanosensors and were imaged using SERS microspectroscopy. We also demonstrate the applicability of SERS mapping for high-throughput multiplex detection using micropatterned cell arrays

Publisher: SPIE - The International Society for Optical Engineering
Year: 2012
DOI identifier: 10.1117/12.906419
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Provided by: Enlighten
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