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Inhibitor of cysteine peptidase does not influence the development of <i>Leishmania mexicana</i> in <i>Lutzomyia longipalpis</i>

By Lucie Jecna, Anna Svarovska, Sebastien Besteiro, Jeremy C. Mottram, Graham H. Coombs and Petr Volf

Abstract

It has been proposed that the natural cysteine peptidase inhibitor ICP of <i>Leishmania mexicana</i> protects the protozoan parasite from insect host proteolytic enzymes, thereby promoting, survival. To test this hypothesis <i>L. mexicana</i> mutants deficient in ICP were evaluated for their ability to develop in the sand fly <i>Lutzomyia longipalpis</i>. No significant differences were found between the wild-type parasites, two independently derived ICP-deficient mutants, or mutants overexpressing ICP all lines developed similarly in the sand fly midgut and produced heavy late-stage infections. In addition, recombinant <i>L. mexicana</i> ICP did not inhibit peptidase activity of the midgut extracts in vitro. We conclude that ICP has no major role in promoting survival of <i>L. mexicana</i> in the vectorial part of its life cycle in <i>L. longipalpis</i

Topics: QR355, QH345
Publisher: Entomological Society of America -
Year: 2009
DOI identifier: 10.1603/033.046.0327
OAI identifier: oai:eprints.gla.ac.uk:6383
Provided by: Enlighten
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