CAPILLARY ZONE ELECTROPHORESIS IONSPRAY MASS-SPECTROMETRY OF A SYNTHETIC DRUG PROTEIN CONJUGATE MIXTURE

Abstract

Low-molecular-mass proteins, such as lysozyme, may be suitable carriers to target drugs to the kidney. Naproxen, an anti-inflammatory drug, has been conjugated with lysozyme via a covalent amide bond formed between the carboxylic acid function of naproxen and the amino group of one of the lysines in lysozyme. The reaction products were analysed by capillary electrophoresis-ionspray mass spectrometry. Native lysozyme and its conjugates with one, two and three naproxen molecules were separated and their identities were confirmed by mass spectrometry. The ion current profiles of the individual conjugates showed pH-dependent tailing and adsorption-desorption phenomena in the capillary electrophoresis column not observed in the total ion current profiles and not observable by UV detection

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