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Phenotypic identification of Candida albicans by growth on chocolate agar.

By Chirag C. Sheth, Elizabeth Johnson, Mark E. Baker, Ken Haynes and Fritz A. Mühlschlegel

Abstract

In this study, we describe a simple method for the identification of Candida albicans in clinical samples. A total of 383 clinical isolates of Candida species were streaked onto chocolate agar and incubated for 48 h at 37 degrees C in the presence of an atmosphere of 6% CO2. All 208 of the C. albicans isolates tested, developed an easy to identify filamentous colony morphology. Of 175 other Candida species tested, 172 (98.3%) were distinguishable from C. albicans by their smooth colony morphology. Three isolates (1.7%) exhibited weak filamentation after prolonged incubation. Although not a routine medium in medical mycology a significant advantage of using chocolate agar lies in its use in clinical bacteriology laboratories for the isolation of fastidious bacteria. Implementation of the proposed method is applicable across a range of specimen types, thus allowing the direct identification of C. albicans in clinical samples. This simple method may allow a quicker entry into directed treatment

Topics: Q
Publisher: Oxford Journals
Year: 2005
DOI identifier: 10.1080/13693780500265998
OAI identifier: oai:kar.kent.ac.uk:50
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