Article thumbnail

The possible role of ChemR23/Chemerin axis in the recruitment of dendritic cells in lupus nephritis.

By Giuseppe De Palma, Giuseppe Castellano, Annalisa Del Prete, Silvano Sozzani, Nicoletta Fiore, Antonia Loverre, Marc Parmentier, Loreto Gesualdo, Giuseppe Grandaliano and Francesco P Schena

Abstract

Dendritic cells (DCs) have a pivotal role in the autoimmune response of systemic lupus erythematosus. Plasmacytoid DCs infiltrate the kidney of patients with lupus nephritis, but factors regulating their recruitment to the kidney are unknown. Chemerin is the recently identified natural ligand of ChemR23, a receptor highly expressed by plasmacytoid DCs. We performed immunohistochemical and immunofluorescence analysis to study the ChemR23/Chemerin axis in renal biopsies from patients with lupus nephritis. We found ChemR23-positive DCs had infiltrated the kidney tubulointerstitium in patients with severe lupus nephritis. Chemerin association with tubular epithelial cells and renal lymphatic endothelial cells was found in patients with lupus nephritis but not in normal kidneys. Proximal tubular epithelial cells produced Chemerin in vitro, which was significantly down-modulated by added tumor necrosis factor (TNF)-α and interferon-γ as measured by quantitative PCR and enzyme-linked immunosorbent assay. Interestingly, TNF-α was capable of inducing a functionally active form of renal Chemerin, resulting in an efficient transendothelial migration of plasmacytoid DCs measured in transwell systems. Thus, the ChemR23/Chemerin axis may have a role in the recruitment of DCs within the kidney in patients affected by lupus nephritis.Journal ArticleResearch Support, Non-U.S. Gov'tSCOPUS: ar.jinfo:eu-repo/semantics/publishe

Topics: Pharmacologie moléculaire et cellulaire, Néphrologie - urologie, Immunologie, Biopsy, Case-Control Studies, Cells, Cultured, Chemokines -- genetics -- metabolism, Chemotaxis, Coculture Techniques, Dendritic Cells -- immunology, Endothelial Cells -- immunology, Enzyme-Linked Immunosorbent Assay, Epithelial Cells -- immunology -- pathology, Fluorescent Antibody Technique, Humans, Immunohistochemistry, Interferon-gamma -- metabolism, Kidney -- immunology -- pathology, Kidney Tubules, Proximal -- immunology -- pathology, Lupus Nephritis -- immunology -- pathology, RNA, Messenger -- metabolism, Receptors, Chemokine -- metabolism, Reverse Transcriptase Polymerase Chain Reaction, Severity of Illness Index, Signal Transduction, Time Factors, Transendothelial and Transepithelial Migration, Tumor Necrosis Factor-alpha -- metabolism, chemokine, chemokine receptor, lupus nephritis, systemic lupus erythematosus
Publisher: 'Springer Science and Business Media LLC'
Year: 2011
DOI identifier: 10.1038/ki.2011.32
OAI identifier: oai:dipot.ulb.ac.be:2013/114495
Provided by: DI-fusion
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://hdl.handle.net/2013/ULB... (external link)

  • To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

    Suggested articles