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Facilitation of insulin release by N-p-tosylglycine

By Abdullah Sener, Ramon Gomis, Bernard Billaudel and Willy Malaisse

Abstract

N-p-tosylglycine, which inhibits transglutaminase activity in islet homogenates, was found to cause a rapid and sustained facilitation of insulin release evoked by D-glucose, L-leucine or the association of Ba2+ and theophylline in intact islets. Such a facilitating action could not be attributed to any obvious effect upon either nutrient oxidation or 45Ca net uptake and outflow. It failed to be reproduced by glycine, Nα-p-tosyl-L-arginine methyl ester or Nα-p-tosyl-L-lysine methyl ester. N-p-tosylglycine (5.0 mM) slightly enhanced insulin release evoked by a high concentration of glucose (16.7 mM) and failed to affect significantly the secretory response to the association of L-leucine and L-glutamine or that of D-glucose and gliclazide. N-p-tosylglycine failed to affect the incorporation of [2,5-3H]histamine in trichloroacetic acid-precipitable material in intact islets. These results suggest that N-p-tosylglycine interferes with a late event in the secretory sequence, possibly at the level of the cell boundary, rather than inhibiting the crosslinking of intracellular proteins.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Topics: Sciences bio-médicales et agricoles
Publisher: 'Elsevier BV'
Year: 1985
DOI identifier: 10.1016/0006-2952(85)90532-5
OAI identifier: oai:dipot.ulb.ac.be:2013/79287
Provided by: DI-fusion
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