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A monoclonal antibody to Ly-6 gene product inhibits generation of functionally active T cells and recognizes single antigenic specificity whose expression is up-regulated in virus-transformed rat fibroblast

By A Haque, Mostafa Chamekh, Jan Cornelis, André Capron and S Haque

Abstract

In order to elucidate the relationship between the structure and function of proteins encoded for by the Ly-6 gene complex, a cDNA was constructed for a Ly-6.2 specificity and then monoclonal antibodies (mAb) generated to bacterially synthesized protein. The addition of one of these mAb, designated Pb-19, inhibited the proliferative response of T cells to concanavalin A (Con A) or major histocompatability complex (MHC) alloantigens. Reactivity of Pb-19 to the Ly-6 specificity was blocked by a known anti-Ly-6.A.2 mAb but not by an anti-Ly-6.E.1 mAb. This mAb detected a Ly-6.A.2 specificity (a 33,000 MW antigen) whose expression was increased in a transformed rat fibroblast containing the entire genome of bovine papillomavirus.Journal ArticleResearch Support, Non-U.S. Gov'tFLWNOinfo:eu-repo/semantics/publishe

Topics: Sciences bio-médicales et agricoles, Animals, Antibodies, Monoclonal -- pharmacology, Antigens, CD27, Antigens, Differentiation, T-Lymphocyte -- genetics, Antigens, Differentiation, T-Lymphocyte -- immunology, Antigens, Ly -- genetics, Antigens, Ly -- immunology, Cell Line, Transformed, Epitopes -- immunology, Fibroblasts -- immunology, Lymphocyte Activation -- immunology, Membrane Proteins -- genetics, Membrane Proteins -- immunology, Rats, Receptors, Antigen, T-Cell -- genetics, Receptors, Antigen, T-Cell -- immunology, T-Lymphocytes -- immunology, Up-Regulation -- immunology
Year: 1990
OAI identifier: oai:dipot.ulb.ac.be:2013/50519
Provided by: DI-fusion
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