Skip to main content
Article thumbnail
Location of Repository

Infrared linear dichroism spectroscopy on amyloid fibrils aligned by molecular combing

By Jose C. Rodriguez-Perez, Ian W. Hamley and Adam M. Squires

Abstract

We report the use of molecular combing as an alignment method to obtain macroscopically oriented amyloid\ud fibrils on planar surfaces. The aligned fibrils are studied by polarized infrared spectroscopy. This gives structural information that cannot be definitively obtained from standard infrared experiments on isotropic samples, for example, confirmation of the characteristic cross-beta amyloid core structure, the side-chain orientation\ud from specific amino acids, and the arrangement of the\ud strands within the fibrils, as we demonstrate here. We employed amyloid fibrils from hen egg white lysozyme (HEWL) and from a model octapeptide. Our results demonstrate molecular combing as a straightforward method to align amyloid fibrils, producing highly anisotropic infrared linear dichroism (IRLD) spectra

Publisher: American Chemical Society
Year: 2011
OAI identifier: oai:centaur.reading.ac.uk:19775
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://dx.doi.org/10.1021/bm20... (external link)
  • http://www.reading.ac.uk/chemi... (external link)
  • http://www.reading.ac.uk/chemi... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.