Skip to main content
Article thumbnail
Location of Repository

Glutathione-S-Transferase and Thiol Stress in patients with acute renal failure

By M Prakash, V Kedage, MS Muttigi, K Nataraj, WW Baig and RP Attur

Abstract

Introduction: Tubular damage is common finding in acute renal failure (ARF). Various etiologies have been put forth to explain the tubular damage in ARF, one important mechanism among them is oxidative damage to renal tubules. Several biomolecules including low-molecular weight peptides and enzymes in urine have been proposed as early markers of renal failure. Current study has been undertaken to study the thiol stress and glutathione-S-transferase (GST) levels in ARF patients. Method: 58 ARF patients and 55 healthy controls were selected based on inclusion and exclusion criteria. Serum thiols, GST, malanoldehyde (MDA) and urine thiols were determined by spectrophotometer based methods. Results: Serum thiols and urine thiols were significantly decreased (p<0.0001), and serum GST and MDA levels were significantly increased (p<0.0001) in ARF patients compared to healthy controls. Serum GST and MDA correlated positively in ARF cases (r2 = 0.6938, p<0.0001). Conclusion: There is significant thiol stress and increased lipid peroxidation in ARF patients which leads to tubular cell membrane damage and release of GST into blood stream and into urine. This may be possible mechanism for the increased presence of GST in urine (enzymuria) found in other studie

Topics: Online Journal of Health and Allied Sciences
Publisher: Online Journal of Health and Allied Sciences
Year: 2010
OAI identifier: oai:cogprints.org:7009

Suggested articles

Citations

  1. (1996). Acute renal failure.
  2. (1988). Antioxidant defenses and lipid peroxidation in human blood plasma.
  3. (1993). Antioxidant protection against hypochlorous acid in human plasma.
  4. Apoptotic pathways in ischemic acute renal failure.
  5. (2002). Beta-scission of sidechain alkoxyl radicals on peptides and proteins results in the loss of side-chains as aldehydes and ketones. Free Radic Biol Med
  6. Binding of heme by Glutathiones- S Transferases – A possible role of erythrocyte enzyme.
  7. Biomarkers of acute renal injury and renal failure.
  8. Effect of an altered glutathione content on renal ischemic injury.
  9. Effects of antioxidants on oxidant-induced sister chromatid exchange formation. doi
  10. (1986). Endotoxemic acute renal failure in awake rats.
  11. (1988). Evidence against oxidant injury as a critical mediator of postischemic acute renal failure.
  12. Glutathione depletion sensitizes tumor cells to oxidative cytolysis.
  13. Glutathione-STranferases: The first enzymatic step in mercapturic acid formation.
  14. Increased superoxide radical formation evokes inducible DNA repair in Escherichia coli.
  15. (1985). Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance.
  16. (1994). Measurement of Antioxidants in Human blood plasma: Protein Thiols. In:
  17. Measurement of tubular enzymuria facilitates early detection of acute renal impairment in the intensive care unit.
  18. (1990). Metal ion-catalyzed oxidation of proteins: Biochemical mechanism and biological consequences. Free Radic Biol Med
  19. Molecular mechanisms of acute renal failure following ischemia/reperfusion.
  20. Noninvasive renal diagnostic studies.
  21. Oxidation of proteins in rat heart and lungs by polymorphonuclear leukocyte oxidants. doi
  22. Oxidative Stress Is Increased in Critically Ill Patients with Acute Renal Failure.
  23. (2002). Oxidized amino acids: Culprits in human atherosclerosis and indicators of oxidative stress. Free Radic Biol Med
  24. (1991). Oxygen free radicals in acute renal failure. Miner Electrolyte Metab
  25. Protein thiol oxidation and lipid peroxidation in patients with uremia.
  26. Renal work, glutathione and susceptibility to free radical-mediated postischemic injury.
  27. (2002). Role of oxidative stress and protein oxidation in the aging process. Free Radic Biol Med
  28. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method.
  29. Stable markers of oxidant damage to proteins and their application in the study of human disease. Free Radic Biol Med 1999;27:1151–1163.
  30. (1990). The antioxidants of human extracellular fluids.
  31. (1990). The effect of glutathione content on renal function following warm ischemia.
  32. (1990). Urinary proteins of tubular origin: basic immunological and clinical aspects.
  33. (1999). Urine N-acetyl-β-D-glucosaminidase - a marker of tubular damage?

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.