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A Study on L-Asparaginase of Nocardia levis MK-VL_113

By Alapati Kavitha and Muvva Vijayalakshmi

Abstract

An enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature 30°C. Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase by N. levis

Topics: Research Article
Publisher: The Scientific World Journal
OAI identifier: oai:pubmedcentral.nih.gov:3349320
Provided by: PubMed Central
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