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Impact of Environmental Thermal Stimulation on Activation of Hypothalamic Neuronal Nitric Oxide Synthase during the Prenatal Ontogenesis in Muscovy Ducks

By Valery Dunai and Barbara Tzschentke


The aim of the study is to investigate the influence of prenatal temperature stimulation on neuronal NO synthase (nNOS) expression in the anterior hypothalamus of Muscovy duck embryos. Experiments were performed on embryonic day (E) E20, E23, E28, and E33 using histochemistry for identification of the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) as marker of NOS-containing neurons. Until the experiments, all duck embryos were incubated under standard temperature conditions (37.5°C). During 3 hours before the start of the experiments, one group was incubated at 37.5°C (control group), the second was warm-experienced at 39°C, and the third was cold-experienced at 34°C. In normal and warm-incubated duck embryos, nNOS activity could be first detected on E23. Particularly, after cold stimulation, a significant increase in nNOS activity was found in all embryos investigated even on day 20. Warm stimulation obviously induces the opposite effect, but at later embryonic age (E33). It can be concluded that probably in late-term bird embryos NO acts as a mediator of the neuronal cold pathway in the anterior hypothalamus, which might be improved by prenatal cold stimulation

Topics: Research Article
Publisher: The Scientific World Journal
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Provided by: PubMed Central

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  1. (1987). a k a s h i m a ,F .K .P i e r a u ,E .S i m o n ,a n dT .H o r i ,“ C o m p a r -ison between hypothalamic thermoresponsive neurons from duck and rat slices,” Pflugers Archiv European
  2. (1993). A role for nitric oxide in active thermoregulatory vasodilation,”
  3. (1998). A Stereotaxic Atlas of the Brain of the Chick (Gallus domesticus), The John Hopkins
  4. (1993). a t s u m o t o ,M .N a k a n e ,J .S .P o l l o c k ,J .E .K u k ,a n d U. Forstermann, “A correlation between soluble brain nitric oxide synthase and NADPH-diaphorase activity is only seen after exposure of the tissue to fixative,”
  5. (2002). Antipyretic role of the NO-cGMP pathway in the anteroventral preoptic region of the rat brain,”
  6. (2009). Asymmetric spike-timing dependent plasticity of striatal nitric oxidesynthase interneurons,”
  7. (2007). Attainment of thermoregulation as affected by environmental factors,”
  8. (2009). Carnio et al., “Role of brain nitric oxide in the thermoregulation of broiler chicks,”
  9. (1999). Central action of nitric oxide in the saltwater-acclimated duck: modulation of extrarenal sodium excretion and vasotocin release,”
  10. (1997). Central application of a nitric oxide donor activates heat defense in the rabbit,”
  11. (2004). Characteristics of early development of body functions and epigenetic adaptation to the environment in poultry: focused on development of central nervous mechanisms,”
  12. (1996). Comparative distribution of NADPH-diaphorase activity and tyrosine hydroxylase immunoreactivity in the diencephalon and mesencephalon of the domestic chicken (Gallus domesticus),”
  13. (2008). Concepts of neural nitric oxide-mediated transmission,”
  14. (1983). Demonstration of a unique population of neurons with NADPH-diaphorase histochemistry,”
  15. (1999). Development of avian thermoregulatory system during the early postnatal period: development of the thermoregulatory set-point,”
  16. (2000). Development of hypothalamic neuronal thermosensitivity in birds during the perinatal period,”
  17. (2004). Development-related changes in the expression of shear stress responsive genes KLF-2, ET-1, andNOS-3inthedevelopingcardiovascularsystemofchicken embryos,” DevelopmentalDynamics,
  18. (1991). E.DeB lasio ,andA.M.English,“N(G)-M o no meth ylL-arginineco-injectionattenuatesthethermogenicandhyperthermic effects of E2 prostaglandin microinjection into the anterior hypothalamic preoptic area in rats,”
  19. (2002). Early development of neuronal hypothalamic thermosensitivity in birds: influence of epigenetic temperature adaptation,”
  20. (1999). Effect of the NO synthase inhibitor, L-NAME, on body temperature in birds in different periods of postnatal ontogenesis,”
  21. (2011). Embryonic development of endothermy,”
  22. (1999). Evidence that nitric oxide stimulates feeding
  23. (2004). Heat acclimation affects the neuromodulatory role of AngII and nitric oxide during combined heat and hypohydration stress,”
  24. (1989). Histochemical characterization of neuronal NADPH-diaphorase,”
  25. (1994). Hypothalamic network for thermoregulatory shivering,”
  26. Hypothalamic network for thermoregulatory vasomotor control,”
  27. (1977). Hypothalamic thermoregulatory pathways in the rat,”
  28. (1998). i c h e l m a n n ,A .B u r m e i s t e r ,O .J a n k e
  29. (2006). Imprinting and critical periods in early development,”
  30. (2000). Incubation physiology,” in Sturkey’s Avian Physiology,
  31. (2009). Influence of temperature stimulation during the last 4 days of incubation on secondary sex ratioandlaterperformanceinmaleandfemalebroilerchicks,”
  32. (2003). Inhibitors of nitric oxide synthesis block cold-induced thermogenesis in rats,”
  33. (2007). Interaction of leptin and nitric oxide on food intake
  34. (2008). Involvement of hippocampal nitric oxide in spatial learning in the rat,”
  35. (1993). Localization of NADPH-diaphorase in
  36. (2010). Long-lasting effect of changes in incubation temperature on heat stress induced neuronal hypothalamicc-Fosexpressioninchickens.SpecialIssue:early development and epigenetic programming of body functions in birds (Ed.
  37. (2002). Metabolic responses of chicken and muscovy duck embryos to high incubation temperatures,”
  38. (2008). Monitoring the development of thermoregulation in poultry embryos and its influence by incubation
  39. (2009). Neurochemistry of the paraventricular nucleus of the hypothalamus: implications for cardiovascular regulation,”
  40. (1994). Nitric oxide controls feeding behavior in the chicken,”
  41. (1992). Nitric oxide controls oscillatory activity in thalamocortical neurons,” Neuron,v o l .9 ,n o .3 ,p p .
  42. (1995). Nitric oxide in the nervous system,”
  43. (2005). Nitric oxide pathway is an important modulator of heat loss in rats during exercise,”
  44. (1995). Nitric oxide signaling in the central nervous system,”
  45. (2008). Nitric oxide synthase inhibitor attenuates the anorexigenic effect of corticotropin-releasinghormoneinneonatalchicks,”Comparative
  46. (2009). Nitric oxide-mediated epigenetic mechanisms in developing neurons,”
  47. (2006). nNOS is involved in behavioral thermoregulation of newborn rats during hypoxia,”
  48. Nomenclature and location of avian hypothalamic nuclei and associated circumventricular organs,”
  49. (2002). O l d fi e l d ,M .E .G i l e s ,A .W a t s o n ,C .A n d e r s o n
  50. (1994). o r n ,P .M .S m i t h ,B .E .M c L a u g h l i ne ta l . ,“ N i t r i c oxide actions in paraventricular nucleus: cardiovascular and neurochemical implications,”
  51. (2003). On the role of nitric oxide in hippocampallong-termpotentiation,”JournalofNeuroscience,
  52. (2002). Ontogeny of thermoregulation in precocial birds,”
  53. (2007). Peripheral or central administration of nitric oxide synthaseinhibitoraffectsfeedingbehaviorinchicks,”Comparative Biochemistry and Physiology,
  54. (2004). Possible role of nitric oxide emission from bird embryos,”
  55. (2004). Prenatal development of epigenetic adaptation processes in poultry: changes
  56. (2004). Reduced ventromedial hypothalamic neuronal nitric oxide synthase and increased sensitivity to NOS inhibition in dietary obese rats: further6 The Scientific World Journal evidence of a role for nitric oxide in the regulation of energy balance,”
  57. (1994). Role of nitric oxide in lipopolysaccharideinduced fever in conscious rabbits,”
  58. (1993). Temperature sensitivity of neurons in slices of the rat PO/AH area: effect of bombesin and substance
  59. (1991). The early development of thermoregulation in birds,”
  60. (1999). Thermoregulation in precocial avian embryos,” Ornis Fennica,v o l .7 6 ,n o .4 ,p p .
  61. (2003). Thermoregulation in rats during early postnatal maturation: importance of nitric oxide,”
  62. (2008). z y f ,P .M c G o w a n ,a n dM .J .M e a n e y ,“ T h es o c i a le n v i -ronment and the epigenome,” Environmental and Molecular Mutagenesis,
  63. (2007). α-Synuclein involvement in hippocampal synaptic plasticity: role of NO, cGMP, cGK and CaMKII,”

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