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Coronin 1C harbours a second actin-binding site that confers co-operative binding to F-actin

By Keefe T. Chan, David W. Roadcap, Nicholas Holoweckyj and James E. Bear

Abstract

Dynamic rearrangement of actin filament networks is critical for cell motility, phagocytosis and endocytosis. Coronins facilitate these processes, in part, by their ability to bind F-actin (filamentous actin). We previously identified a conserved surface-exposed arginine (Arg30) in the β-propeller of Coronin 1B required for F-actin binding in vitro and in vivo. However, whether this finding translates to other coronins has not been well defined. Using quantitative actin-binding assays, we show that mutating the equivalent residue abolishes F-actin binding in Coronin 1A, but not Coronin 1C. By mutagenesis and biochemical competition, we have identified a second actin-binding site in the unique region of Coronin 1C. Interestingly, leading-edge localization of Coronin 1C in fibroblasts requires the conserved site in the β-propeller, but not the site in the unique region. Furthermore, in contrast with Coronin 1A and Coronin 1B, Coronin 1C displays highly co-operative binding to actin filaments. In the present study, we highlight a novel mode of coronin regulation, which has implications for how coronins orchestrate cytoskeletal dynamics

Topics: Research Article
Publisher: Portland Press Ltd.
OAI identifier: oai:pubmedcentral.nih.gov:3339874
Provided by: PubMed Central
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    1. (2005). A conserved trimerization motif controls the topology of short coiled coils.
    2. (2003). A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference.
    3. (2007). activities at the leading edge.
    4. (2005). Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules.
    5. B by protein kinase C regulates interaction with Arp2/3 and cell motility.
    6. (2005). Cofilin binding to muscle and non-muscle actin filaments: isoform-dependent cooperative interactions.
    7. (1997). Cofilin changes the twist of F-actin: implications for actin filament dynamics and cellular function.
    8. (2009). Cofilin dissociates Arp2/3 complex and branches from actin filaments.
    9. (1980). Cooperative binding of myosin subfragment-1 to the actin–troponin–tropomyosin complex.
    10. (1979). Cooperative binding of tropomyosin to muscle and Acanthamoeba actin.
    11. (1996). Cooperativity in F-actin: binding of gelsolin at the barbed end affects structure and dynamics of the whole filament.
    12. (2008). Coronin 1B antagonizes cortactin and remodels Arp2/3-containing actin branches in lamellipodia.
    13. (2007). Coronin 3 involvement in F-actin-dependent processes at the cell cortex. Exp. Cell Res. 313, 878–895 c The Authors Journal compilation c 2012 Biochemical Society ©
    14. (1999). Coronin localizes to leading edges and is involved in cell spreading and lamellipodium extension in vertebrate cells.
    15. (1999). Coronin promotes the rapid assembly and cross-linking of actin filaments and may link the actin and microtubule cytoskeletons in yeast.
    16. (2009). Coronin switches roles in actin disassembly depending on the nucleotide state of actin.
    17. (2008). Coronin-1A stabilizes F-actin by bridging adjacent actin protomers and stapling opposite strands of the actin filament.
    18. (1991). Coronin, an actin binding protein of Dictyostelium discoideum localized to cell surface projections, has sequence similarities to G-protein β subunits.
    19. (2006). Coronins: the return of the crown. Trends Cell Biol.
    20. (2005). Cortactin promotes cell motility by enhancing lamellipodial persistence.
    21. (2011). Differential regulation of mast cell degranulation versus cytokine secretion by the actin regulatory proteins Coronin1a and Coronin1b.
    22. (2007). Enhanced EGFP-chromophore-assisted laser inactivation using deficient cells rescued with functional EGFP-fusion proteins.
    23. (2008). Expression of coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy.
    24. (2007). F-actin binding is essential for coronin 1B function in vivo.J .C e l lS c i .120,
    25. (2010). Functional surfaces on the actin-binding protein coronin revealed by systematic mutagenesis.
    26. (2005). Homotypic dimerization of the actin-binding protein p57/coronin-1 mediated by a leucine zipper motif in the C-terminal region.
    27. (2000). Isolation and chromosomal assignment of a novel human gene, CORO1C, homologous to coronin-like actin-binding proteins.
    28. lymphocyte trafficking and cellular homeostasis.
    29. (2011). Mechanism of a concentration-dependent switch between activation and inhibition of Arp2/3 complex by coronin.
    30. (2000). Negative regulation of fibroblast motility by Ena/VASP proteins.
    31. (2002). Oligomerization, F-actin interaction, and membrane association of the ubiquitous mammalian coronin 3 are mediated by its carboxyl terminus.
    32. (2005). Phosphorylation of coronin
    33. (2010). Proteome of acidic phospholipid-binding proteins: spatial and temporal regulation of Coronin 1A by phosphoinositides.
    34. (2006). Rapid actin monomer-insensitive depolymerization of Listeria actin comet tails by cofilin, coronin, and Aip1.
    35. (2006). Requirement for coronin
    36. (2006). The crystal structure of murine coronin-1: a regulator of actin cytoskeletal dynamics in lymphocytes.
    37. (2008). The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines.
    38. (2006). The identification of a new actin-binding region in p57. Cell Res.
    39. (2003). Two regions responsible for the actin binding of p57, a mammalian coronin family actin-binding protein.
    40. (2011). Unraveling the enigma: progress towards understanding the coronin family of actin regulators.
    41. (2010). WD40 proteins propel cellular networks.

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