Article thumbnail

A novel osteogenesis technique: The expansible guided bone regeneration

By Osama Zakaria, Marwa Madi and Shohei Kasugai

Abstract

Guided bone regeneration is a unique osteogenesis technique that requires a barrier membrane under periosteum to create space for bone regeneration. However, creating sizeable spaces is clinically not commonly feasible. A titanium plate and a thin silicone membrane were surgically layered on each calvaria of eight rabbits. Then, the periphery of the silicone membrane was fixed by a plastic ring to the underlying bone using titanium micro screws. After 1 week, a 5-mm-length titanium screw was used to elevate the titanium plate, which in turn elevated the silicone membrane together with overlying soft tissue in a rate of 1 mm/day for 5 days to create a secluded space. Animals were killed at 2 months (n = 4, group 1) and 4 months (n = 4, group 2) after the elevation. Histological and microradiographical analyses demonstrated creation of an amount of de novo bone formation (68.2 ± 22 mm3 in group 1 and 70.3 ± 14 mm3 in group 2) in the sizeable created spaces (207.1 ± 31 mm3 in group 1 and 202 ± 21 mm3 in group 2) without exposure of the device. This novel osteogenesis technique, “expansible guided bone regeneration,” created a substantial in vivo incubator without applying growth factors or osteoprogenitor cells. Creating a growing space over the secluded surface allowed the development of normal biological healing process occurring on the bone surface into a regenerative process, generating bone outside the genetically determined skeletal bone. This technique is a new tissue engineering approach stimulating endogenous tissue repair without applying cells or factors exogenously

Topics: Article
Publisher: SAGE Publications
OAI identifier: oai:pubmedcentral.nih.gov:3324854
Provided by: PubMed Central

Suggested articles

Citations

  1. (1994). A preliminary report on a method for studying the permeability of expanded polytetrafluoroethylene membrane to bacteria in vitro: a scanning electron microscopic and histological study.
  2. (1999). Augmentation of calvarial tissue using nonpermeable silicone domes and bovine bone mineral—an experimental study in the rat. Clin Oral Implants Res
  3. (1995). Augmentation of intramembranous bone beyond the skeletal envelope using an occlusive titanium barrier. An experimental study in the rabbit. Clin Oral Implants Res
  4. (1995). Bacterial penetration in-vitro through GTAM membrane with and without topical chlorhexidine application. A light and scanning electron-microscopic study.
  5. (2008). Basic principles of scaffolds in tissue engineering. Scaffolds for tissue engineering and regenerative medicine.
  6. (1997). Blood-filled spaces with and without filler materials in guided bone regeneration. A comparative experimental study in the rabbit using bioresorbable membranes [Comparative Study Research Support, Non-US Government]. Clin Oral Implants Res
  7. (2003). Cambium cell stimulation from surgical release of the periosteum [Research Support,
  8. Clinical case reports of injectable tissue-engineered bone for alveolar augmentation with simultaneous implant placement [Case Reports Research Support, Non-US Government]. Int J Periodontics Restorative Dent 2005; 25(2): 129–137.Zakaria et al. 9
  9. (2000). Contribution of the periosteum to bone formation in guided bone regeneration. A study in monkeys. Clin Oral Implants Res
  10. (2008). Cortical bone thickness in the buccal posterior region for orthodontic miniimplants [Research Support, Non-US Government]. Int J Oral Maxillofac Surg
  11. (1993). Creation of new bone by an osteopromotive membrane technique: an experimental study in rats.
  12. (2007). Dynamic periosteal elevation.
  13. (2009). Effects of cortical bone perforation on periosteal distraction: an experimental study in the rabbit mandible [Comparative Study Research Support, Non-US Government]. J Oral Maxillofac Surg
  14. (1996). Effects of different osteopromotive membrane porosities on experimental bone neogenesis in rats. Biomaterials
  15. (2003). Effects of occlusiveness of a titanium cap on bone generation beyond the skeletal envelope in the rabbit calvarium [Research Support, Non-US Government]. Clin Oral Implants Res
  16. (2012). Evaluation of a biodegradable novel periosteal distractor.
  17. (1993). Expression of growth factors during healing of rat mandibular trephine lesions treated by the osteopromotive membrane technique.
  18. (2007). Facilitated endogenous repair: making tissue engineering simple, practical, and economical [Research Support, N.I.H., Extramural Review]. Tissue Eng
  19. (1994). Guided bone regeneration in mandibular defects in rats using a bioresorbable polymer. Clin Oral Implants Res
  20. (1994). Healing pattern of bone regeneration in membrane-protected defects: a histologic study in the canine mandible [Comparative Study Research Support, Non-US Government]. Int J Oral Maxillofac Implants
  21. (2007). Histomorphometrical analysis of new bone obtained by distraction osteogenesis and osteogenesis by periosteal distraction in rabbits [Comparative Study Evaluation Studies]. Int J Oral Maxillofac Surg
  22. (2005). In situ osteogenesis: regeneration of 10-cm mandibular defect in porcine model using recombinant human bone morphogenetic protein-2 (rhBMP2) and helistat absorbable collagen sponge. J Craniofac Surg
  23. (2005). In vivo engineering of organs: the bone bioreactor [Comparative Study Research Support, Non-US Government].
  24. (2002). Induced osteogenesis by periosteal distraction [Research Support, Non-US Government]. J Oral Maxillofac Surg
  25. (2012). Induced osteogenesis using a new periosteal distractor.
  26. (2000). Influence of decortication of the donor bone on guided bone augmentation. An experimental study in the rabbit skull bone [Research Support, Non-US Government]. Clin Oral Implants Res
  27. (2006). Lateral compartment osteoarthritis of the knee after meniscectomy treated by the transplantation of tissue-engineered cartilage and osteochondral plug [Case Reports]. Arthroscopy
  28. (2005). Long-term stability of autogenous bone grafts following combined application with guided bone regeneration. Clin Oral Implants Res
  29. (2004). Osteochondral lesion located at the lateral femoral condyle reconstructed by the transplantation of tissue-engineered cartilage in combination with a periosteum with bone block: a case report. Knee Surg Sports Traumatol Arthrosc
  30. Periosteal cells in bone tissue engineering [Review]. Tissue Eng 2003; 9(Suppl 1):
  31. (2011). Periosteal distraction osteogenesis and barrier membrane application: an experimental study in the rat calvaria.
  32. (2007). Periosteal distraction osteogenesis: preliminary experimental evaluation in rabbits and dogs [Evaluation Studies]. Br J Oral Maxillofac Surg
  33. (2004). Platelet-rich plasma: evidence to support its use.
  34. (1995). Temporal dynamics of healing in rabbit cranial defects using guided bone regeneration. J Oral Maxillofac Surg
  35. (1997). The biological effect of natural bone mineral on bone neoformation on the rabbit skull. Clin Oral Implants Res
  36. (2009). The effect of periosteal injury and masticatory micromovement on the healing of a mandibular distraction osteogenesis site [Research Support, N.I.H., Extramural Research Support, Non-US Government]. Arch Oral Biol
  37. (2006). The efficacy of various bone augmentation procedures for dental implants: a Cochrane systematic review of randomized controlled clinical trials. Int J Oral Max Impl
  38. (2004). Tissue engineering: the end of the beginning. Tissue Eng
  39. (2008). VEGF facilitates periosteal distraction-induced osteogenesis in rabbits: a micro-computerized tomography study. Tissue Eng Part A
  40. (2005). Why tissue engineering needs process engineering.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.