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Ribosome Distribution in HeLa Cells during the Cell Cycle

By Yuan-Jhih Tsai, Hsing-I Lee and Alan Lin

Abstract

In this study, we employed a surface-specific antibody against the large ribosome subunit to investigate the distribution of ribosomes in cells during the cell cycle. The antibody, anti-L7n, was raised against an expansion segment (ES) peptide from the large subunit ribosomal protein L7, and its ribosome-surface specificity was evident from the positive immuno-reactivity of ribosome particles and the detection of 60 S immune-complex formation by an immuno-electron microscopy. Using immunofluorescent staining, we have microscopically revealed that ribosomes are dispersed in the cytoplasm of cells throughout all phases of the cell cycle, except at the G2 phase where ribosomes show a tendency to gather toward the nuclear envelope. The finding in G2 cells was confirmed by electron microscopy using a morphometric assay and paired t test. Furthermore, further observations have shown that ribosomes are not distributed immune-fluorescently with nuclear envelope markers including the nuclear pore complex, the integral membrane protein gp210, the inner membrane protein lamin B2, and the endoplasm reticulum membrane during cell division we propose that the mechanism associated with ribosome segregation into daughter cells could be independent of the processes of disassembly and reassembly of the nuclear envelope

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:3293906
Provided by: PubMed Central
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    1. (1993). 180-kD ribosome receptor is essential for both ribosome binding and protein translocation.
    2. (2007). A
    3. (2000). A comparison of the yeast and rabbit 80 S ribosome reveals the topology of the nascent chain exit tunnel, inter-subunit bridges and mammalian rRNA expansion segments.
    4. (2001). A role for nuclear lamins in nuclear envelope assembly.
    5. (2000). An encore for ribosome biogenesis in the control of cell proliferation.
    6. (2010). Dark-field light scattering imaging of living cancer cell component from birth through division using bioconjugated gold nanoprobes.
    7. (2007). Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells.
    8. (1986). Identification and characterization of a nuclear pore complex protein.
    9. (1991). Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts.
    10. (1990). Insulin and step-up conditions cause a redistribution of polysomes among free, cytoskeletal-bound and membranebound fractions in Krebs II ascites cells.
    11. (2009). Knockdown of p180 eliminates the terminal differentiation of a secretory cell line.
    12. (2008). Lin A
    13. (1991). Localization of surface peptide from ribosomal protein L7 on 80 S ribosome by biotinylation.
    14. (2003). Nuclear pore protein gp210 is essential for viability in HeLa cells and Caenorhabditis elegans.
    15. (2008). Ribosomal RNAs are tolerant toward genetic insertions: evolutionary origin of the expansion segments.
    16. (2006). Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane.
    17. (2007). Ribosome biogenesis is sensed at the Start cell cycle checkpoint.
    18. (1994). Ribosome synthesis during the growth cycle of Saccharomyces cerevisiae.
    19. (2010). Single cell nanoparticle tracking to model cell cycle dynamics and compartmental inheritance.
    20. (2011). Statistical analysis of nanoparticle dosing in a dynamic cellular system.
    21. (1989). Synthesis of ribosomes in Saccharomyces cerevisiae.
    22. (1999). The economics of ribosome biosynthesis in yeast.
    23. (1984). The localization of protein L19 on the surface of 50 S subunits of Escherichia coli aided by the use of mutants lacking protein L19.
    24. (1983). The partitioning of cytoplasmic organelles at cell division.
    25. (1998). Translocation of 60 S ribosomal subunit in spreading cardiac myocytes.

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