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Detection of Infectious Poxvirus Particles

By Andreas Nitsche, Daniel Stern, Heinz Ellerbrok and Georg Pauli

Abstract

To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription–polymerase chain reaction detection of 2 viral genes expressed immediately after infection

Topics: Dispatch
Publisher: Centers for Disease Control and Prevention
OAI identifier: oai:pubmedcentral.nih.gov:3291062
Provided by: PubMed Central

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Citations

  1. Detection of orthopoxvirus DNA by real-time PCR and identification of variola virus DNA by melting analysis.
  2. (2006). Emerging Infectious Diseases •

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