Article thumbnail

The Inheritance of Histone Modifications Depends upon the Location in the Chromosome in Saccharomyces cerevisiae

By Hiroshi Masumoto, Ryuichiro Nakato, Masato Kanemaki, Katsuhiko Shirahige and Mayumi Hachinohe


Histone modifications are important epigenetic features of chromatin that must be replicated faithfully. However, the molecular mechanisms required to duplicate and maintain histone modification patterns in chromatin remain to be determined. Here, we show that the introduction of histone modifications into newly deposited nucleosomes depends upon their location in the chromosome. In Saccharomyces cerevisiae, newly deposited nucleosomes consisting of newly synthesized histone H3-H4 tetramers are distributed throughout the entire chromosome. Methylation of lysine 4 on histone H3 (H3-K4), a hallmark of euchromatin, is introduced into these newly deposited nucleosomes, regardless of whether the neighboring preexisting nucleosomes harbor the K4 mutation in histone H3. Furthermore, if the heterochromatin-binding protein Sir3 is unavailable during DNA replication, histone H3-K4 methylation is introduced onto newly deposited nucleosomes in telomeric heterochromatin. Thus, a conservative distribution model most accurately explains the inheritance of histone modifications because the location of histones within euchromatin or heterochromatin determines which histone modifications are introduced

Topics: Research Article
Publisher: Public Library of Science
OAI identifier:
Provided by: PubMed Central

Suggested articles


  1. (2002). Active genes are tri-methylated at K4 of histone H3.
  2. (1998). Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae.
  3. (2009). An auxin-based degron system for the rapid depletion of proteins in nonplant cells.
  4. (2003). An epigenetic road map for histone lysine methylation.
  5. (2006). Cell cycle and checkpoint regulation of histone
  6. (2007). Chromatin challenges during DNA replication and repair.
  7. (2007). Chromatin modifications and their function.
  8. (1999). Chromatin: Structure and Function, 3rd Ed.
  9. (2006). Chromosomal association of the Smc5/6 complex reveals that it functions in differently regulated pathways.
  10. (2002). Chromosomal gradient of histone acetylation established by Sas2p and Sir2p functions as a shield against gene silencing.
  11. (1989). Elevated recombination rates in transcriptionally active DNA.
  12. (2005). Epigenetic regulation by histone methylation and histone variants.
  13. (2005). Genome-wide map of nucleosome acetylation and methylation in yeast.
  14. (2003). Histone and chromatin cross-talk.
  15. (2004). Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis.
  16. (2004). Histone variants, nucleosome assembly and epigenetic inheritance.
  17. (1997). Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs.
  18. (2004). Methylation of H3 lysine 4 at euchromatin promotes Sir3p association with heterochromatin.
  19. (2009). Multiple histone modifications in euchromatin promote heterochromatin formation by redundant mechanisms in Saccharomyces cerevisiae.
  20. (1992). Nonconservative segregation of parental nucleosomes during simian virus 40 chromosome replication in vitro.
  21. (2002). Ordered nucleation and spreading of silenced chromatin in Saccharomyces cerevisiae.
  22. (2010). Partitioning of histone H3-H4 tetramers during DNA replication-dependent chromatin assembly.
  23. (2011). Patterns and mechanisms of ancestral histone protein inheritance in budding yeast.
  24. (2001). Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
  25. (1997). SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast.
  26. (2007). Sir2 deacetylates histone H3 lysine 56 to regulate telomeric heterochromatin structure in yeast.
  27. (2005). Split decision: what happens to nucleosomes during DNA replication?
  28. (2011). Splitting of H3-H4 tetramers at transcriptionally active genes undergoing dynamic histone exchange.
  29. (1986). Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures.
  30. (1988). Sugino A
  31. (2003). Targeted recruitment of Set1 histone methylase by elongating Pol II provides a localized mark and memory of recent transcriptional activity.
  32. (2008). The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes.
  33. (1997). The efficiency and timing of initiation of replication of multiple replicons of Saccharomyces cerevisiae chromosome VI.
  34. (2003). The establishment, inheritance, and function of silenced chromatin in Saccharomyces cerevisiae.
  35. (2006). The sirtuins Hst3p and Hst4p preserve genome integrity by controlling histone H3 lysine 56 deacetylation.
  36. (1999). Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.
  37. (2005). Verreault A

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.