Article thumbnail

Direct Electrochemistry and Electrocatalysis of Hemoglobin at Mesoporous Carbon Modified Electrode

By Supeng Pei, Song Qu and Yongming Zhang

Abstract

The novel highly ordered mesoporous carbon (known as FDU-15), prepared by the organic-organic self-assembly method was been used for first time for the immobilization of hemoglobin (Hb) and its bioelectrochemical properties were studied. The resulting Hb/FDU-15 film provided a favorable microenvironment for Hb to perform direct electron transfers at the electrode. The immobilized Hb also displayed its good electrocatalytic activity for the reduction of hydrogen peroxide. The results demonstrate that mesoporous carbon FDU-15 can improve the Hb loading with retention of its bioactivity and greatly promote the direct electron transfer, which can be attributed to its high specific surface area, uniform ordered porous structure, suitable pore size and biocompatibility. Our present study may provide an alternative way for the construction of nanostructure biofunctional surfaces and pave the way for its application to biosensors

Topics: Article
Publisher: Molecular Diversity Preservation International (MDPI)
OAI identifier: oai:pubmedcentral.nih.gov:3244013
Provided by: PubMed Central

Suggested articles

Citations

  1. (2005). A facile aqueous route to synthesize highly ordered mesoporous polymer and carbon frameworks with Ia-3d bicontinuous cubic structure.
  2. (2009). A promising electrochemical biosensing platform based on graphitized ordered mesoporous carbon.
  3. (2008). A voltammetric biosensor based on glassy carbon electrodes modified with single-walled carbon nanotubes/hemoglobin for detection of acrylamide in water extracts from potato crisps. Sensors
  4. (2005). Achieving direct electrical connection to glucose oxidase using aligned single walled carbon nanotube arrays. Electroanalysis
  5. (2009). Applications of nanomaterials in electrochemical enzyme biosensors. Sensors
  6. (2007). Biosensing paraoxon in simulated environmental samples by immobilized organophosphorus hydrolase in functionalized mesoporous silica.
  7. (1999). Carbon materials for lithium ion rechargeable batteries. Carbon
  8. (2005). Coadsorption of horseradish peroxidase with thionine on TiO2 nanotubes for biosensing. Langmuir
  9. (2005). Direct electrochemistry and electrocatalysis of heme proteins immobilized on self-assembled
  10. Direct electrochemistry and electrocatalysis of hemoglobin immobilized in bimodal mesoporous silica and chitosan inorganic-organic hybrid film.
  11. (1998). Direct electrochemistry of cofactor redox sites in a bacterial photosynthetic reaction center protein.
  12. (2002). Direct electrochemistry of cytochrome c at a glassy carbon electrode modified with single-wall carbon nanotubes.
  13. Direct electrochemistry of cytochrome c on a multi-walled carbon nanotubes modified electrode and its electrocatalytic activity for the reduction of H2O2.
  14. (2002). Direct electrochemistry of hemoglobin in egg-phosphatidylcholine films and its catalysis to H2O2.
  15. (2000). Direct electrochemistry of hemoglobin in polyacrylamide hydrogel films on pyrolytic graphite electrodes. Electroanalysis
  16. (2010). Direct electron transfer and bioelectrocatalysis of hemoglobin at a carbon nanotube electrode.
  17. (2004). Direct electron transfer and enzymatic activity of hemoglobin in a hexagonal mesoporous silica matrix.
  18. (2001). Direct electron transfer for heme proteins assembled on nanocrystalline
  19. Encapsulation hemoglobin in ordered mesoporous silicas: Influence factors for immobilization and bioelectrochemistry.
  20. (1980). General expression of the linear potential sweep voltammogram for a surface redox reaction with interactions between the adsorbed molecules: Applications to modified electrodes.
  21. (2008). Highly ordered mesoporous carbons as electrode material for the construction of electmchemical dehydrogenase- and oxidase-based biosensors.
  22. (2009). Immobilization of HRP in mesoporous silica and its application for the construction of polyaniline modified hydrogen peroxide biosensor. Sensors
  23. (2000). Integration of layered redox proteins and conductive supports for bioelectronic applications.
  24. (1998). Novel nanocarbons-structure, properties, and potential applications.
  25. (2008). Ordered mesopomus carbon paste electrodes for electrochemical sensing and biosensing. Electroanalysis
  26. (2005). Ordered mesoporous polymers and homologous carbon frameworks: amphiphilic surfactant templating and direct transformation.
  27. Reporting physisorption data for gas/solid systems with special reference to the determination of surface area and porosity.
  28. (1980). Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer.
  29. (1999). Synthesis of highly ordered carbonmolecular sieves via template-mediated structuraltransformation.
  30. (2005). Synthesis of ordered mesoporous carbons with channel structure from an organic-organic nanocomposite.
  31. (2004). Synthesis of ordered, uniform, macroporous carbons with mesoporous walls templated by aggregates of polystyrene spheres and silica particles for use as catalyst supports in direct methanol fuel cells.
  32. (2005). The interface behavior of hemoglobin at carbon nanotube and the detection for H2O2. Talanta
  33. (1979). The use of linear potential sweep voltammetry and of a.c. voltammetry for the study of the surface electrochemical reaction of strongly adsorbed systems and of redox modified electrodes.
  34. (1998). Triblock Copolymer Syntheses of mesoporous silica with periodic 50 to 300 angstrom pores. Science
  35. (1999). Ultrasonic Deposition of high-selectivity nanoporous carbon membranes. Science

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.