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Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen▿†

By Fabien Miszczak, Kathleen M. Shuck, Zhengchun Lu, Yun Young Go, Jianqiang Zhang, Stephen Sells, Astrid Vabret, Stéphane Pronost, Guillaume Fortier, Peter J. Timoney and Udeni B. R. Balasuriya

Abstract

This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen

Topics: Clinical Veterinary Microbiology
Publisher: American Society for Microbiology
OAI identifier: oai:pubmedcentral.nih.gov:3187312
Provided by: PubMed Central
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