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Building and Using MS/MS Spectral Libraries for Peptide Identifications in Proteomics

By H. Lam

Abstract

Spectral library searching is an emerging approach in peptide identifications from tandem mass spectra, a critical step in proteomic data analysis. In this approach, a spectral library is first meticulously compiled from a large collection of previously observed and identified peptide MS/MS spectra. An unknown spectrum is then identified by comparing it to all the candidates in the spectral library for the most similar match. Thanks to the reduction of search space to only the previously discovered peptides, and the use of real, experimentally observed reference spectra for more precise spectral matching, this approach is considerably faster and more sensitive than the popular alternative of sequence searching. This talk will explain the basic principles of spectral library building and searching, describe its advantages and limitations, and provide a starting point for researchers interested in adopting this new approach in their data analysis. It will also discuss the future outlook on the evolution and utility of spectral libraries in the field of proteomics

Topics: Workshop Session Abstracts
Publisher: Association of Biomolecular Resource Facilities
OAI identifier: oai:pubmedcentral.nih.gov:3186628
Provided by: PubMed Central
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