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Assembling Complete Genomes from Complex Mixtures

By F. Meyer

Abstract

Characterizing genomes of un-culturable microbes (most species on earth) requires new approaches for genome assembly from environmental or biomedical samples that often contain many hundreds or thousands of species. Here we show we can reconstruct the complete genome (Candidatus Sulfuricurvum sp) via short-read metagenomics and novel approaches for assembly based on simple statistical principles. While previous examples of complete genome sequences from metagenomes stem from samples of very limited complexity (>10 OTUs), this sequence was obtained from a complex mix of over 300 OTUs. The reason traditional genome assembly approaches fail are varying abundance levels and strain variation and we show a simple approach to overcome those hurdles. This novel approach will allow the assembly of genomes and via metabolic models derived from the genomic sequences, hopefully the cultivation of key species from diverse environments/enrichment cultures

Topics: Scientific Session Abstracts
Publisher: Association of Biomolecular Resource Facilities
OAI identifier: oai:pubmedcentral.nih.gov:3186539
Provided by: PubMed Central
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