Article thumbnail

Effects of incomplete decay in fluorescence lifetime estimation

By Regina Won Kay Leung, Shu-Chi Allison Yeh and Qiyin Fang

Abstract

Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can superimpose onto the subsequent decay measurements. Using a mathematical model, the incomplete decay effect has been shown to lead to overestimation of the amplitude average lifetime except in mono-exponential decays. An inverse model is then developed to correct the error from this effect and the theoretical simulations are tested by experimental results

Topics: Spectroscopic Diagnostics
Publisher: Optical Society of America
OAI identifier: oai:pubmedcentral.nih.gov:3184861
Provided by: PubMed Central

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.