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The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model

By Egija Zaura, Mark J. Buijs, Michel A. Hoogenkamp, Lena Ciric, Adele Papetti, Caterina Signoretto, Monica Stauder, Peter Lingström, Jonathan Pratten, David A. Spratt and Michael Wilson


The aim of the current study was to investigate the anticariogenic potential of the (sub)fractions obtained from the edible mushroom shiitake (Lentinula edodes) in in vitro caries model. We used a modified constant depth film fermentor (CDFF) with pooled saliva as the inoculum and bovine dentin as a substratum. The test compounds were low molecular weight fraction (MLMW) of the shiitake extract and subfractions 4 and 5 (SF4 and SF5) of this fraction. Chlorhexidine (CHX) and water served as a positive and a negative control, respectively. Dentin mineral loss was quantified (TMR), microbial shifts within the microcosms were determined (qPCR), and the acidogenicity of the microcosms was assessed (CIA). From the compounds tested, the SF4 of shiitake showed strong inhibiting effect on dentin demineralization and induced microbial shifts that could be associated with oral health. The acid producing potential was increased, suggesting uncoupling of the glycolysis of the microbiota by the exposure to SF4. In conclusion, the results suggest that SF4 of shiitake has an anticariogenic potential

Topics: Research Article
Publisher: Hindawi Publishing Corporation
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Provided by: PubMed Central

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  4. (1993). Antimicrobial strategies in the prevention of dental caries,”
  5. (2008). Antimicrobialactivityofextractsofediblewildandcultivated mushrooms against foodborne bacterial strains,”
  6. (2003). C.J.vanNevel,J.A.Decuypere,N.Dierick,andK.Molly,“The influenceoflentinusedodes(Shiitakemushroom)preparations on bacteriological and morphological aspects of the small intestine in piglets,”
  7. (2005). Cariespreventive agents induce remineralization of dentin in a biofilm model,”
  8. (2002). Communication among oral bacteria,”
  9. (1998). Composition and susceptibility to chlorhexidine of multispecies biofilms of oral bacteria,”
  10. (2010). Development of a novel multi-triplex qPCR method for the assessment of bacterial community structure in oral populations,”
  11. (1979). Effect of chlorhexidine on acidogenicity of dental plaque in vivo,”
  12. Evaluation of plant and fungal extracts for their anti-gingivitis and anti-caries activity,”
  13. (2006). G .A .v a nd e rW e i j d e n ,a n dJ .M .t e nC a t e ,“ E ffects of various rinsing protocols after the use of amine fluoride/stannous fluoride toothpaste on the acid production of dental plaque and tongue flora,”
  14. (2007). Growing oral biofilms in a constant depth film fermentor
  15. (2003). Growth and molecular characterization of dental plaque microcosms,”
  16. (1998). i l s o n ,H .P a t e l ,a n dJ .H .N o a r ,“ E ffect of chlorhexidine on multi-species biofilms,”
  17. (1988). Introduction,” in Handbook of Laboratory Model Systems for Microbial Ecosystems,
  18. (1993). oev en,D .C ummins,M.J .Schaek en,andF .J .van der Ouderaa, “The effect of chlorhexidine and zinc/triclosan mouthrinses on the production of acids in dental plaque,”
  19. (2000). Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNAand rRNA-based microbial community composition,”
  20. (2002). ten Cate, “Acidogenicity of buccal plaque after a single rinse with amine fluoridestannous fluoride mouthrinse solution,”
  21. (2004). ten Cate, “Demineralization of dentin by Streptococcus mutans biofilms grown in the constant depth film fermentor,”
  22. (2007). ten Cate, “Effects of ozone and sodium hypochlorite on caries-like lesions in dentin,”
  23. (1994). ten Cate, “The video camera compared with the densitometer as a scanning device for microradiography,”
  24. (2009). The resident oral microflora,”
  25. (1976). Uncoupling of growth and acid production in lactic streptococci,”
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