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A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions

By Charles W. Sokolik, Annie S. Walker and Gary M. Nishioka

Abstract

This study describes an extremely sensitive and simple assay to measure small volumes of solutions, <1 nL. The assay takes advantage of the Sandell-Kolthoff reaction in which yellow cerium(IV) is reduced to colorless cerium(III) in the presence of arsenic(III) and catalytic quantities of iodide ion. The reaction is linear with respect to the rate of Ce(IV) reduction and the quantity of I− present. Typical assays can measure 10–100 picomoles of iodide in a sample. When I− is substituted for chloride ion in standard biological buffers, such as Tris-buffered saline, the assay can be used to determine the volume of solution printed in a microarray

Topics: Rapid Communication
Publisher: Libertas Academica
OAI identifier: oai:pubmedcentral.nih.gov:3169343
Provided by: PubMed Central

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Citations

  1. An Addressable Antibody Nanoarray Produced on a Nanostructured Surface.
  2. (1969). Analytical Applications of the Iodide and Osmium Catalyzed Reaction between Cerium(IV) and Arsenic(III). Anal Chem.
  3. Behavior of Microscopic Droplets Near a Strong Electrostatic Field: Droplet Electrospray. Analytical Chemistry.
  4. (2007). Borrebaeck CAK. Progress in Miniaturization of Protein Arrays—A Step Closer to High-Density Nanoarrays. Drug Discovery Today.
  5. (2002). Improving Performance in Protein-Based Microarrays.
  6. (1969). Kinetics of the Iodide-Catalyzed Reaction between Cerium(IV) and Arsenic(III) in Sulfuric Acid Medium. Anal Chem.
  7. (1937). Micro Determination of Iodine by a Catalytic Method. Microchim Acta.
  8. (2001). On-Demand Droplet Spotter for Preparing Pico- to Femtoliter Droplets on Surfaces. Analytical Chemistry.
  9. On-demand mixing droplets spotter for preparing picoliter droplets on surfaces. Analytical Chemistry.
  10. Physical and Chemical Studies of a Limited Reaction of Iodine with Proteins.
  11. Printing Chemical Libraries on Microarrays dor Fluid Phase Nanoliter Reactions.
  12. Protein and Antibody Microarray Technology.
  13. Protein Nanoarrays Generated By Dip-Pen Nanolithography.
  14. Simple Microplate Method for Determination of Urinary Iodine. Clinical Chem.