Article thumbnail

Genetic variation among species, races, forms and inbred lines of lac insects belonging to the genus Kerria (Homoptera, Tachardiidae)

By Sanjeev Kumar Ranjan, Chandana Basu Mallick, Dipnarayan Saha, Ambarish S Vidyarthi and Ranganathan Ramani


The lac insects (Homoptera: Tachardiidae), belonging to the genus Kerria, are commercially exploited for the production of lac. Kerria lacca is the most commonly used species in India. RAPD markers were used for assessing genetic variation in forty-eight lines of Kerria, especially among geographic races, infrasubspecific forms, cultivated lines, inbred lines, etc., of K. lacca. In the 48 lines studied, the 26 RAPD primers generated 173 loci, showing 97.7% polymorphism. By using neighbor-joining, the dendrogram generated from the similarity matrix resolved the lines into basically two clusters and outgroups. The major cluster, comprising 32 lines, included mainly cultivated lines of the rangeeni form, geographic races and inbred lines of K. lacca. The second cluster consisted of eight lines of K. lacca, seven of the kusmi form and one of the rangeeni from the southern state of Karnataka. The remaining eight lines formed a series of outgroups, this including a group of three yellow mutant lines of K. lacca and other species of the Kerria studied, among others. Color mutants always showed distinctive banding patterns compared to their wild-type counterparts from the same population. This study also adds support to the current status of kusmi and rangeeni, as infraspecific forms of K. lacca

Topics: Evolutionary Genetics
Publisher: Sociedade Brasileira de Genética
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (1998). A and Krishan Sharma K
  2. (1958). A Catalogue of the Lac Insects (Lacciferidae,
  3. (2006). A comparison of the intraspecific variability of Phlebotomus sergenti Parrot,
  4. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars.
  5. (2000). A new trivoltine species of Kerria Targioni-Tozzetti (Homoptera,
  6. (1985). A review of Indian Coccids (Homoptera, Coccoidea). Oriental Insects 19:1-101. VarshneyRK(2009)Revisedsynopticcatalogueofthelacinsects of the world
  7. (1923). A systematic monograph of the Tachardiinae or lac insects (Coccidae).
  8. (1994). Analysis of population genetic structure with RAPD markers.
  9. (1992). Applications of random amplified polymorphic DNA (RAPD) in molecular ecology.
  10. BaldacchinoF,SimeoneVandMalacridaAR(2007)RAPD analysis in the parasitoid wasp Psyttalia concolor reveals Mediterranean population structure and provides SCAR markers.
  11. (1987). Demographic study of a wild house sparrow population by DNA fingerprinting.
  12. (2007). Diffusion of the Nearctic leafhopper Scaphoideus titanus Ball in Europe: A consequence of human trading activity.
  13. (1994). DNA fingerprinting in rice using oligonucleotide probes specific for simple repetitive DNA sequences.
  14. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals.
  15. (1990). Fingerprinting genomes using PCR with primers.
  16. (1977). Gene expression and transmission in Kerria lacca (Kerr).
  17. (1970). Genetic evidence of nutritional differences in lac insects.
  18. (2005). Genetics of lac insects. In:
  19. (2010). Jaiswal AK and Bhattacharya A (2011) Lac statistics at a glance
  20. (2007). Lac – An Introduction.
  21. (1950). Lac and the Lac Insect in the Atharva-Veda.
  22. (1962). Lac Hosts. In:
  23. (2002). Lac insect genetics. In:
  24. (2005). Molecular characterization and relatedness of Haematobia irritans (horn fly) populations, by RAPD-PCR.
  25. (2006). Molecular marker systems in insects: Current trends and future avenues.
  26. (1998). NTSYS-PC: Numerical taxonomy and multivariate analysis system,
  27. (1973). Numerical Taxonomy.
  28. ParasharBD,VijayVeer,AgarwalOPandPrakashS(2009) Genetic variability in geographical populations of Culex quinquefasciatus Say (Diptera, Culicidae) from India based on random amplified polymorphic DNA analysis.
  29. (1995). Polymorphism within and between populations of Ceratitis capitata: Comparison between RAPD and multilocus enzyme electrophoresis data.
  30. (2007). Population structure of the banana weevil, an introduced pest in the Canary Islands, studied by RAPD analysis.
  31. (1995). populations of the grain aphid Sitobion avenae (F.) (Hemiptera, Aphididae) studied using RAPD-PCR.
  32. (2008). RAPD and mitochondrial DNA analysis of the soybean stalk weevil, Sternechus subsignatus (Coleoptera,
  33. (2003). Retnakaran A and Feng Q
  34. (1925). Supplement to a monograph on the Lacciferidae (Tachardiinae) or lac insects
  35. (1977). Taxonomic studies of lac insects of India (Homoptera, Tachardiidae). Oriental Insects Suppl
  36. (1962). The lac insect. In:
  37. (1987). The neighbor-joining method: A new methodforreconstructingphylogenetictrees.MolBiolEvol 4:406-425. SarkarPC(2002)Applicationsoflac–Past,presentandemerging trends. In:
  38. (1998). Use of molecular markers for detecting the geographical origin of Ceratitis capitata populations.
  39. (1977). White: A new colour locus in Kerria lacca (Kerr) Curr Sci