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Folliculostellate cells in pituitary pars distalis of male viscacha: immunohistochemical, morphometric and ultrastructural study

By M. Acosta, V. Filippa and F. Mohamed


Folliculostellate cells (FSC) have been reported in pituitary of several mammalian species. FSC morphology and secreted substances have been instrumental to the understanding of their function. The purpose of this work was to perform an immunohistochemical, morphometric and ultrastructural study of the pituitary pars distalis FSC in adult male viscacha and to analyze their relation with hormone secreting cells. Immunohistochemistry and image analysis were carried out in different sectors of the gland, from the middle (sector 1) to the glandular periphery (sector 5). Transmission electron microscopy with lanthanum as electrodense tracer was used. FSC formed follicles with PAS-positive colloid inside. They expressed S-100 protein mainly in both nucleus and cytoplasm. FSC were stellate-like in shape and exhibited short cytoplasmic processes that contacted with blood vessels and endocrine cells. In addition, some follicular colloids were immunostained with anti-S-100 protein. A few FSC were immunostained with anti-glial fibrillary acidic protein (GFAP) and anti-vimentin. The morphometric parameters analyzed (percentages of S-100-positive total, cellular and colloidal areas) increased from sector 1 to sector 3 and then decreased to sector 5. Hormone secreting cells, mainly lactotrophs, gonadotrophs and corticotrophs were associated with FSC and follicles. The ultrastructural study demonstrated that FSC developed junctional complexes and desmosomes between their lateral membranes. Lanthanum freely penetrated the spaces between granulated cells and FSC, but did not penetrate into the follicular lumen. In conclusion: 1) the differential expression of S-100 protein, GFAP and vimentin may indicate different physiological stages of FSC; 2) the expression of these proteins suggests a neuroectodermic origin of these cells; 3) FSC spatial distribution, association with endocrine cells, and the generation of an intercellular communication network suggest that FSC are involved in the pituitary pars distalis paracrine regulation of the viscacha

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  3. ACTH cells of pituitary pars distalis of viscacha (Lagostomus maximus maximus): immunohistochemical study in relation to season, sex, and growth.
  4. An insight to pituitary folliculo-stellate cells.
  5. (1993). and group fusion in a declining population of the plains vizcacha (Lagostomus maximus;
  6. (2002). Are folliculo-stellate cells in the anterior pituitary gland supportive cells or organ-specific stem cells? Arch Physiol Biochem
  7. Biochemical and morphological diversity among folliculo-stellate cells of the mink (Mustela vison) anterior pituitary.
  8. Calcium-dependent and – independent interactions of the S100 protein family.
  9. Changes in the population of immunoreactive S-100-positive folliculo-stellate cells in hens durOriginal paper[European Journal of Histochemistry 2010; 54:e1] [page 9] ing induced molting.
  10. Colloid in the anterior pituitary of helmet guinea fowl (Numida meleagris galeata): morphometric analysis and pattern of occurrence in relation to apoptosis.
  11. (2000). Colloid in the pituitary pars distalis of viscacha (Lagostomus maximus maximus): ultrastructure and occurrence in relation to season, sex, and growth. Anat Rec
  12. (1998). Comparative immunohistochemical study of stellate cells in normal canine and equine adenohypophyses and in pituitary tumors.
  13. (1954). Ecología de la vizcacha (Lagostomus maximus maximus) en el nordeste de la provincia de Entre Ríos. Rev Investig Agric. Extra Nueva Serie
  14. Epididymis of viscacha (Lagostomus maximus maximus): morphological changes during the annual reproductive cycle. Anat Rec
  15. Extracellular spaces of the rat pars intermedia as outlined by lanthanum tracer.
  16. Folliculostellate cells of the pituitary.
  17. (1967). Hexagonal array of subunits in intercellular junctions of the mouse heart and liver.
  18. (1996). Histochemical study of follicles in the senescent porcine pituitary gland. Arch Histol Cytol
  19. History and perspectives of pituitary folliculo-stellate cell research.
  20. Immunocytochemical localization of basic fibroblast growth factor in the rat pituitary gland.
  21. (1989). Immunocytochemical localization of vimentin in stellate cells (folliculo-stellate cells) of the rat, cat and rabbit pituitary pars distalis. Anat Embryol
  22. (2008). Immunohistochemical and morphometirc study of pituitary pars distalis thyrotrophs of male viscacha (Lagostomus maximus maximus): seasonal variations and effect of melatonin and castration. Anat Rec
  23. (1989). Immunohistochemical localization of intermediate filament and S-100 proteins in several non-endocrine cells of the human pituitary gland. Arch Histol Cytol
  24. Immunohistochemical localization of S-100 protein in the pig pituitary gland.
  25. (2006). Immunohistochemical study of somatotrophs in pituitary pars distalis of male viscacha (Lagostomus maximus maximus) in relation to the gonadal activity. Cells Tissues Organs
  26. (1994). Immunohistochemical study of the post-natal development of the folliculo-stellate cells in the rat anterior pituitary gland. Tissue Cell
  27. Intercellular communications within the rat anterior pituitary XI: an immunohistochemical study of distributions of S-100 positive cells in the anterior pituitary of the rat.
  28. (1990). Intermediate filament expression in nonneoplastic pituitary cells.
  29. (1996). Lagostomus maximus.
  30. (1992). Mammals of the neotropics: The southern cone. Vol 2.
  31. Marginal cells of the viscacha pituitary cleft. A correlate study by thin-sectioning, lanthanum intercellular tracer and freezefracture techniques.
  32. (2009). Morphological and morphometric changes of pituitary lactotrophs of viscacha (Lagostomus maximus maximus) in relation to reproductive cycle, age, and sex. Anat Rec Forthcoming
  33. Novel clonal strains from adult rat anterior pituitary producing S-100 protein.
  34. Occurrence of colloid-containing follicles in the pars distalis of pituitary glands from aging guinea pigs.
  35. (1990). Permeability of pineal gland of viscacha (Lagostomus maximus maximus) to the lanthanum hydroxide.
  36. Phagocytotic removal of apoptotic endocrine cells by folliculostellate cells and its functional implications in clusterin accumulation in pituitary colloids in helmeted guinea fowl (Numida meleagris).
  37. (2009). Pituitary pars intermedia of male viscacha (Lagostomus maximus maximus): a morphometric study of seasonal and age-related changes in immunohistochemistry. Cells Tissues Organs.
  38. (1974). possible function and fate of “follicular cells” in the anterior lobe of the human pituitary.
  39. Regulatory activity and topological distribution of folliculostellate cells in rat anterior pituitary aggregates.
  40. S-100 protein in folliculostellate cells of the rat pituitary anterior lobe.
  41. S-100: a multigenic family of calcium-modulated proteins of the EFhand type with intracellular and extracellular functional roles.
  42. (1993). Seasonal changes of the blood-testis in viscacha (Lagostomus maximus maximus): a freese-fracture and lanthanum tracer study. Anat Rec
  43. (1993). Seasonal variations in testicular LH, FSH, and PRL receptors, in vitro testosterone production, and serum testosterone concentration in adult male viscacha (Lagostomus maximus maximus).
  44. (1991). Seasonal variations in the testis and epididymis of viscacha (Lagostomus maximus maximus). Biol Reprod
  45. Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary. Effect of chronic melatonin treatment.
  46. (1991). The fine structure of the S-100 protein positive cells in the rat pituitary gland: an immunoelectron microscopic study. Brain Res
  47. The follicular cell in the pars distalis of the dog pituitary gland: an electron microscope study.
  48. (1997). The glycoproteins that occur in the colloids of senescent porcine pituitary glands are clusterin and glycosylated albumin fragments.
  49. The network of the folliculostellate cells and the follicles of the adenohypophysis in the rat (pars distalis).
  50. The pituitary cleft of the rat: an electron microscopic study.
  51. (2001). Ultrastructural and morphometric study of the Sertoli cell of the viscacha (Lagostomus maximus maximus) during the annual reproductive cycle. Anat Rec