Article thumbnail

Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

By Y.H. Huang, M.J. Sun, M. Jiang and B.Y. Fu


We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PP-secreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells

Topics: Original Paper
Publisher: PAGEPress Publications
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (1995). An immunocytochemical and morphometric study of the rat pancreatic islets.J Anat
  2. Brinster RL,Frohman LA.Immunohistochemical analysis of human growth hormone-releasing hormone gene expression in transgenic mice.
  3. (1980). Distribution and morphometric quantitation of pancreatic endocrine cell types in the frog, Rana pipiens. Anat Rec
  4. (1985). Electron microscopic immunocytochemical localization of glucagon and pancreatic polypeptide in rat pancreas: characterization of a population of islet cells containing both peptides.Anat Rec
  5. (1991). Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene.
  6. (1985). Glucagon and pancreatic polypeptide immunoreactivities co-exist in a population of rat islet cells. Experientia
  7. (1997). Louw J,Woodroof CW,Wolfe-Coote SA.Distribution of endocrine cells displaying immunoreactivity for one or more peptides in the pancreas of the adult vervet monkey (Cercopithecus aethiops).Anat Rec
  8. (1992). Lukinius A,Ericsson JL,Grimelius L,Korsgren O.Ultrastructural studies of the ontogeny of fetal human and porcine endocrine pancreas, with special reference to colocalization of the four major islet hormones.
  9. Miniglucagon (glucagon 19-29): a novel regulator of the pancreatic islet physiology.
  10. (1982). Ontogenic appearance of immunoreactive endocrine cells in rat pancreatic islets. Anatomy and Embryology
  11. (1977). Ontogeny of rat pancreatic polypeptide (PP) cells. Cell Tissue Res
  12. (2008). Origin of pancreatic endocrine cells from biliary duct epithelium. Cell Mol Life Sci
  13. (2006). Oshima H,Taketo MM,Oshima M.Destruction of pancreatic beta-cells by transgenic induction of prostaglandin E2 in the islets.
  14. (1991). Pancreatic endocrine cells in sea bass (Dicentrarchus labrax L.) I. Immunocytochemical characterization of glucagon- and PP-related peptides.
  15. Pancreatic polypeptide islets and glucagon islets: distinct topographic distribution in rat pancreas.
  16. Preliminary observations on the co-existence of regulatory peptides in cells of the baboon endocrine pancreas.
  17. The HMG box transcription factor Sox4 contributes to the development of the endocrine pancreas.
  18. Types of pancreatic islet cells and their immunocytochemical identification.
  19. Vesicular inhibitory amino acid transporter is present in glucagon-containing secretory granules in alphaTC6 cells, mouse clonal alpha-cells, and alpha-cells of islets of Langerhans.
  20. (1999). Voltage-gated and resting membrane currents recorded from B-cells in intact mouse pancreatic islets.J Physiol
  21. Yang L,Li S,Hatch H,Ahrens K,Cornelius JG,Petersen BE,Peck AB. In vitro trans-differentiation of adult hepatic stem cells into pancreatic endocrine hormone-producing cells.
  22. Zhang W,Efanov A,Yang SN,Fried G,Kolare S,Brown H,et al.Munc18 Associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta-cell.