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Cytotoxicity of gamma-ray in rat immature hippocampal neurons

By Miyoung Yang, Myoung-Sub Song, Sung-Ho Kim, Jong-Choon Kim, Joong-Sun Kim, Taekyun Shin and Changjong Moon

Abstract

This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked γ-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of γ-rays in immature hippocampal cultured neurons may be caused by oxidative stress

Topics: Original Article
Publisher: The Korean Society of Veterinary Science
OAI identifier: oai:pubmedcentral.nih.gov:3165147
Provided by: PubMed Central

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