Article thumbnail

Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging

By Dirk Stephan, Coba Slabber, Gavin George, Victor Ninov, Kevin P Francis and Johan T Burger
Topics: Methodology
Publisher: BioMed Central
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (2003). An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.
  2. (1997). Baulcombe DC: A similarity between viral defense and gene silencing in plants. Science
  3. (2010). Bouzoubaa S: P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity.
  4. (2006). Dolja VV: Diverse suppressors of RNA silencing enhance agroinfection by a viral replicon. Virology
  5. (1987). Duplication of CaMV35S promoter sequences creates a strong enhancer for plant genes. Science
  6. (2004). Effects and side-effects of viral RNA silencing suppressors on short RNAs. Trends Plant Sci
  7. (1996). Engineered GFP as a vital reporter in plants. Curr Biol
  8. (2010). EP: High level protein expression in plants through the use of a novel autonomously replicating geminivirus shuttle vector. Plant Biotechnol J
  9. (2003). Fluorescent labelling reveals spatial separation of potyvirus populations in mixed infected Nicotiana benthamiana plants.
  10. Gleba Y: Rapid, high-yield production in plants of individualized idiotype vaccines for non-Hodgkin’s lymphoma. Ann Oncol 2010, online first.
  11. (1998). Initiation and maintenance of virusinduced gene silencing. Plant Cell
  12. (2001). JC: Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system. Plant Physiol
  13. JD: Whole-body imaging of infection using fluorescence. Curr Protoc Microbiol 2011, Chapter 2:Unit 2C.3.
  14. (1999). Jr: Green fluorescent protein as a marker for expression of a second gene in transgenic plants. Nat Biotechnol
  15. (2007). Kuchuk NV: Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotechnol Bioeng
  16. (2005). Laser-induced fluorescence imaging and spectroscopy of GFP transgenic plants.
  17. (2006). Maiss E: Biological properties of Beet mild yellowing virus derived from a full-length cDNA clone.
  18. (2009). Marusic C: High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus.
  19. (2007). Palkovics L: Use of pentapeptide-insertion scanning mutagenesis for functional mapping of the Plum pox virus helper component proteinase suppressor of gene silencing.
  20. (2010). Plant-made vaccines for humans and animals.
  21. (2001). Potyvirus proteins: a wealth of functions. Virus Res
  22. (1994). Prasher DC: Green fluorescent protein as a marker for gene expression. Science
  23. (2008). Quantification and extension of transient GFP expression by the co-introduction of a suppressor of silencing. Transgenic Res
  24. (2004). Quantitative comparison of the sensitivity of detection of fluorescent and bioluminescent reporters in animal models. Mol Imaging
  25. (2009). Quantitative evaluation of six different viral suppressors of silencing using image analysis of transient GFP expression. Plant Cell Rep
  26. (2003). Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants. Plant Cell Rep
  27. (2007). RC: A rapid and sensitive fluorimetric protocol for the quantification of green fluorescent protein in soluble protein extracts from transgenic Arabidopsis. Plant Mol Biol Rep
  28. (1997). Requirement of sense transcription for homology-dependent virus resistance and trans-inactivation. Plant J
  29. (2005). TC: The dark side of green fluorescent protein. New Phytol
  30. (2003). Transitivitydependent and -independent cell-to-cell movement of RNA silencing.
  31. (1995). Tsien RY: Improved green fluorescence. Nature
  32. (2000). Vaucheret H: (Trans)gene silencing in plants: how many mechanisms? Annu Rev Plant Physiol Plant Mol Biol
  33. (2004). VB: Plant viral suppressors of RNA silencing. Virus Res
  34. (2007). Viral vectors for the expression of proteins in plants. Curr Opin Biotechnol
  35. (2009). X: A viral suppressor P1/HCpro increases the GFP gene expression in Agrobacterium-mediated transient assay. Appl Biochem Biotechnol
  36. (2002). Ziegler-Graff V: P0 of Beet western yellows virus is a suppressor of posttranscriptional gene silencing.
  37. (2008). Ziegler-Graff V: Viral suppression of RNA silencing by destabilisation of ARGONAUTE 1. Plant Signal Behav