Article thumbnail

Immunohistochemical localization of autophagosomal membrane-associated protein LC3 in granular cell tumor and schwannoma

By Masayuki Shintaku


Granular cell tumor (GCT) is a neoplasm derived from Schwann cell or (in cases arising in the neurohypophysis) pituicyte and is characterized by abundant cytoplasm filled with numerous eosinophilic granules, which have been considered autophagolysosomes on the basis of their ultrastructure. To confirm that the formation of these granules is related to an autophagy phenomenon, 12 cases of GCT (including two cases of GCT of the neurohypophysis) were studied immunohistochemically using an antibody against LC3 (microtubule-associated protein 1 light chain 3, a specific marker of autophagy). All cases of GCT showed granular immunoreactivity for LC3 in the cytoplasm of tumor cells, indicating that the formation of intracytoplasmic granules in GCT is closely related to an autophagy phenomenon. For elucidation of the relationship between GCT and schwannoma, 20 cases of schwannoma were similarly studied using the anti-LC3 antibody. In eight of 20 cases, a small number of tumor cells showed granular immunoreactivity for LC3, suggesting an increased autophagic activity in some schwannomas and further reinforcing the close relationship between GCT and schwannoma

Topics: Original Article
Publisher: Springer-Verlag
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (1993). Aberrant expression of macrophage-associated antigens (CD68 and KiM1P) by Schwann cells in reactive and neoplastic neural tissue. Light- and electron-microscopic findings.
  2. (2007). Anaplastic medulloblastoma with granular cell change.
  3. (2009). Cell death.
  4. (1976). Eosinophilic granular cells in oligodendrogliomas. An ultrastructural study.
  5. (1994). Granular cell change in smooth muscle tumours of skin and soft tissue.
  6. (1986). Granular cell glioblastoma. A malignant granular cell neoplasm of astrocytic origin.
  7. (1962). Granular cell myoblastoma—a misnomer. Electron microscopic and histochemical evidence concerning its Schwann cell derivation and nature (granular cell schwannoma).
  8. (1971). Granular cell myoblastoma. An electron microscopic and cytochemical study illustrating the genesis of granules and aging of myoblastoma cells.
  9. (1980). Granular cell tumor. A clinicopathologic study of 110 patients.
  10. (2007). Granular cell tumour of the neurohypophysis. In: Louis DN, Ohgaki H, Wiestler OD, Cavenee WK (eds) WHO classification of tumors of the central nervous system, 4th edn. IARC, Lyon,
  11. (1992). Granular cells in a cellular neurilemmoma.
  12. (1994). Immunohistochemical demonstration of the lysosome-associated glycoprotein CD68 (KP-1) in granular cell tumors and schwannomas.
  13. (1983). Immunohistochemical localization of S-100 protein and peripheral nerve myelin proteins (P2 protein, P0 protein) in granular cell tumors.
  14. (1982). Immunohistochemical localization of S-100 protein in granular cell myoblastoma.
  15. (2002). Infiltrative astrocytomas with granular cell features (granular cell astrocytomas). A study of histopathologic features, grading, and outcome.
  16. (1973). Is schwannoma related to granular cell myoblastoma? Arch Pathol 95:396–401
  17. (1992). KP1 (CD68) staining of granular cell neoplasms. Is KP1 a marker for lysosomes rather than the histiocytic lineage?
  18. (2000). LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
  19. (2007). LC3, an autophagosome marker, can be incorporated into protein aggregates independent of autophagy. Caution in the interpretation of LC3 localization.
  20. (1988). Lysosomes in granular cell tumours. Ultrastructural pathology of the cell and matrix, 3rd edn. Butterworths, London,
  21. (2010). Peripheral nerve sheath tumors of the gastrointestinal tract. A multicenter study of 58 patients including NF1-associated gastric schwannoma and unusual morphologic variants.
  22. (1981). The ultrastructure of neurilemoma with emphasis on
  23. (2008). To be or not to be? Examples of incorrect identification of autophagic compartments in conventional transmission electron microscopy of mammalian cells.
  24. (1980). Ultrastructural study of the human neurohypophysis. II. Cellular elements of neural parenchyma, the pituicytes. Cell Tissue Res 205:273–