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Plasma Membrane Domains Participate in pH Banding of Chara Internodal Cells

By Patric M. Schmölzer, Margit Höftberger and Ilse Foissner


We investigated the identity and distribution of cortical domains, stained by the endocytic marker FM 1-43, in branchlet internodal cells of the characean green algae Chara corallina and Chara braunii. Co-labeling with NBD C6-sphingomyelin, a plasma membrane dye, which is not internalized, confirmed their location in the plasma membrane, and co-labelling with the fluorescent pH indicator Lysotracker red indicated an acidic environment. The plasma membrane domains co-localized with the distribution of an antibody against a proton-translocating ATPase, and electron microscopic data confirmed their identity with elaborate plasma membrane invaginations known as charasomes. The average size and the distribution pattern of charasomes correlated with the pH banding pattern of the cell. Charasomes were larger and more frequent at the acidic regions than at the alkaline bands, indicating that they are involved in outward-directed proton transport. Inhibition of photosynthesis by DCMU prevented charasome formation, and incubation in pH buffers resulted in smaller, homogenously distributed charasomes irrespective of whether the pH was clamped at 5.5 or 8.5. These data indicate that the differential size and distribution of charasomes is not due to differences in external pH but reflects active, photosynthesis-dependent pH banding. The fact that pH banding recovered within several minutes in unbuffered medium, however, confirms that pH banding is also possible in cells with evenly distributed charasomes or without charasomes. Cortical mitochondria were also larger and more abundant at the acid bands, and their intimate association with charasomes and chloroplasts suggests an involvement in carbon uptake and photorespiration

Topics: Regular Papers
Publisher: Oxford University Press
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  2. (1965). A cytoplasmic organelle associated with the cell walls of Chara and Nitella.
  3. (1965). An unusual organelle in the peripheral cytoplasm of Chara cells.
  4. (1980). Apparent bicarbonate uptake and possible plasmalemmaprotoneffluxinCharacorallina.PlantPhysiol.66:1198–1199.
  5. (1990). Application of asymmetric alternating voltage pulse series for investigation of the action-potential in Chara.
  6. (2003). Arabidopsis sterol endocytosis involves actin-mediated trafficking via ARA6-positive early endosomes.
  7. (1993). ATP-dependent carbon transport in perfused Chara cells.
  8. (1980). Bicarbonate assimilation by freshwater charophytes and higher-plants. 1. Membranetransport of bicarbonate ions is not proven.
  9. (1991). Calcium–proton exchange during algal calcification.
  10. (2003). Carbon acquisition mechanisms in Chara tomentosa.
  11. (1981). Characean charasomecomplex and plasmalemma vesicle development.
  12. (1989). Charasomes are not essential for photosynthetic utilization of exogenous
  13. (1981). Chloride transport in Chara.1 . Kinetics and current–voltage curves for a probable proton symport.
  14. (1999). Chloromethyltetramethylrosamine (Mitotracker Orange TM) induces the mitochondrial permeability transition and inhibits respiratory complex I. Implications for the mechanism of cytochrome c release.
  15. (2003). Chloroplast movement.
  16. (2001). Comparative study on photosynthetic activity of chloroplasts in acid and alkaline zones of Chara corallina.
  17. (2008). Constitutive endocytosis in characean internodal cells is independent of an intact actin cytoskeleton.
  18. (1983). Cytochemical localisation of ATPase activity on the plasmalemma of Chara corallina.
  19. (1994). Distribution of charasomes in Chara— reestablishment and loss in darkness and correlation with banding and inorganic carbon uptake.
  20. (1991). Distribution of charasomes in Chara—banding-pattern and effect of photosynthetic inhibitors.
  21. (1980). Effects of ammonia and methylamine on Cl transport and on the pH changes and circulating electric currents associated with HCO3 assimilation in Chara corallina.
  22. (1981). Electrogenic ion pumps.
  23. (1965). Electron microscope studies on surface activity in cells of Chara vulgaris.
  24. (1993). Endoplasmic reticulum forms a dynamic continuum for lipid diffusion between contiguous soybean root cells.
  25. (2002). Essentiality of mitochondrial oxidative metabolism for photosynthesis: optimization of carbon assimilation and protection against photoinhibition.
  26. (1980). Evaluation of the polyene antibiotic filipin as a cytochemical probe for membrane cholesterol.
  27. (2009). Extracellular transport and integration of plant secretory proteins into pathogen-induced cell wall compartments.
  28. (2009). Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels.
  29. (2008). FM dyes label sterol-rich plasma membrane domains and are internalized independently of the cytoskeleton in characean internodal cells.
  30. (2004). FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells.
  31. (2001). How characean cells have contributed to the progress of plant membrane biophysics.
  32. (2001). Identification and DNA sequence of a new H +-ATPase in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae).
  33. (1996). Imaging exocytosis and endocytosis.
  34. (1994). Immunolocalization of the plasma membrane H +-ATPase in minor veins of Vicia faba in relation to phloem loading.
  35. (1986). Influence of culture-medium pH on charasome development and chloride transport in Chara corallina.
  36. (1988). Inorganic carbon transport in biological systems.
  37. (1985). Involvement of plasmalemmasomes and carbonic anhydrase in photosynthetic utilization of bicarbonate in Chara corallina.
  38. (2004). Lipid rafts in higher plant cells—purification and characterization of triton X-100-insoluble microdomains from tobacco plasma membrane.
  39. (1969). Localization of hydrogen ion and chloride ion fluxes in Nitella.
  40. (2004). Microfilaments and microtubules control the shape, motility, and subcellular distribution of cortical mitochondria in characean internodal cells.
  41. (1987). Microtubule orientation in developing internodal cells of Nitella—a quantitative-analysis.
  42. (2000). Mitochondria-targeted GFP highlights the heterogeneity of mitochondrial shape, size and movement within living plant cells.
  43. (1989). Molecular cloning and sequence of cDNA encoding the plasma membrane proton pump (H +-ATPase) of Arabidopsis thaliana.
  44. (2006). Neutral red as a probe for confocal laser scanning microscopy studies of plant roots.
  45. (1994). Novel fluorescent acidic organelle-selective dyes and mitochondrion-selective dyes that are well retained during cell fixation and permeabilization.
  46. (1994). Novel site-selective fluorescent probes for lysosome and acidic organelle staining and long-term tracking. Cytometry 18: 77; (abstract 426b).
  47. (1983). Photosynthetic assimilation of exogenous HCO3 by aquatic plants.
  48. (1995). Physiological characteristics and regulation mechanisms of the H + pumps in the plasma membrane and tonoplast of characean cells.
  49. (2003). Plant plasma membrane H + pumps: past and present.
  50. (1970). Plasma membrane domains in Chara and pH bandingLaemmli,
  51. (1996). Plasma membrane isolation from freshwater and salt-tolerant species of Chara: antibody cross-reactions and phosphohydrolase activities.
  52. (1982). Plasmalemma chloride transport in Chara corallina—inhibition by 4,40-diisothiocyano-2,20-disulfonic acid stilbene.
  53. (1994). Relationship between banding and photosynthetic activity in Chara corallina as studied by spatially different induction curves of chlorophyll fluorescene observed by an image analysis system.
  54. (2009). Remorin, a solanaceae protein resident in membrane rafts and plasmodesmata, impairs potato virus X movement.
  55. (1994). Source–sink characteristic of photoassimilate transport in fertile and sterile plants of Chara vulgaris L.
  56. (1977). Spatial distribution of functional OH carriers along a characean internodal cell: determined by the effect of cytochalasin
  57. (2003). Spatio-temporal patterns of photosystem II activity and plasma-membrane proton flows in Chara corallina cells exposed to overall and local illumination.
  58. (1980). Structure and possible function(s) of charasomes; complex plasmalemma–cell wall elaborations present in some characean species.
  59. (1973). The formation of alkaline and acid regions at the surface of Chara corallina cells.
  60. (1982). The mechanism of bicarbonate assimilation by the polar leaves of
  61. (2008). The plant plasma membrane proton pump ATPase: a highly regulated P-type ATPase with multiple physiological roles. Pflugers Arch.
  62. (1982). The relationship of the charasome to chloride uptake in Chara corallina: physiological and histochemical investigations.
  63. (1994). The role of carbonic-anhydrase in photosynthesis.
  64. (1998). Topical aspects of vacuolar protein transport: autophagy and prevacuolar compartments.
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  66. (2004). Use of energy-filtering transmission electron microscopy for routine ultrastructural analysis of high-pressure-frozen or chemically fixed plant cells.