Article thumbnail

Dieckol from Ecklonia cava Regulates Invasion of Human Fibrosarcoma Cells and Modulates MMP-2 and MMP-9 Expression via NF-κB Pathway

By Chen Zhang, Yong Li, Zhong-Ji Qian, Sang-Hoon Lee, Yong-Xin Li and Se-kwon Kim

Abstract

The matrix metalloproteinase (MMP) family is involved in the breakdown of extracellular matrix in normal physiological processes, as well as in the disease processes such as arthritis and cancer metastasis. In the present study, dieckol was obtained with high yield from marine brown alga Ecklonia cava (EC), and its effect was assessed on the expression of MMP-2 and -9 and morphological changes in human fibrosarcoma cell line (HT1080). Dieckol inhibited the expression of MMP-2 and -9 in a dose-dependent manner and also suppressed the cell invasion and the cytomorphology in 3D culture system on HT1080 cells. Moreover, dieckol may influence nuclear factor kappa B (NF-κB) pathway without obvious influence on activator protein-1 (AP-1) pathway and tissue inhibitor of metalloproteinases (TIMPs). In conclusion, dieckol could significantly suppress MMP-2 and -9 expression and alter cytomorphology of HT1080 cell line via NF-κB pathway

Topics: Research Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:3151525
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (2002). A .H .B a k e r ,D .R .E d w a r d s ,a n dG .M u r p h y ,“ M e t a l l o p r o -teinase inhibitors: biological actions and therapeutic opportunities,”
  2. (2005). A cultural renaissance: in vitro cell biology embraces three-dimensional context,”
  3. (2007). A microfluidic device for parallel 3-D cell cultures in asymmetric environments,”
  4. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,”
  5. (1990). Activation in vitro of NF-κBb y phosphorylation of its inhibitor
  6. (2008). Anti-HIV-1 activity of phloroglucinol derivative, 6,6 -bieckol,
  7. (1999). Clinical trials of a matrix metalloproteinase inhibitor in human periodontal disease,”
  8. (2005). Control of melanoma cell invasion by type
  9. (2004). Differential regulation of matrix metalloproteinase-2 and -9 expression and activity in adult rat cardiac fibroblasts in response to interleukin-1β,”
  10. (2004). Drug discovery, CAM and natural products,”
  11. (1983). Human skin fibroblasts collagenase inhibitor. Purification and biochemical characterization,”
  12. (2009). i m ,a n dS .K .K i m , “Inhibitory effects of polyphenols isolated from marine alga Ecklonia cava on histamine release,”
  13. (2006). Inhibition of tumor invasion and metastasis by aqueous extract of the radix of Platycodon grandiflorum,”
  14. (2004). Inhibitory effect of emodin on tumor invasion through suppression of activator protein-1 and nuclear factor-κB,”
  15. (2004). Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation,”
  16. (1999). K¨ ah¨ ari, “Regulation of matrix metalloproteinase expression in tumor invasion,”
  17. (2001). KiSS-1 represses 92-kDa type IV collagenase expression by down-regulating NF-κB binding to the promoter as a consequence of IκBα-induced block of p65/p50 nuclear translocation,”
  18. (2007). M c C h e s n e y ,S .K .V e n k a t a r a m a n ,a n dJ .T .H e n r i ,“ P l a n t natural products: back to the future or into extinction?”
  19. (2003). Marine pharmacology in 2000: antitumor and cytotoxic compounds,”
  20. (1999). Matrix metalloproteinase inhibition during the development of congestive heart failure: effects on left ventricular dimensions and function,”
  21. (2009). Matrix metalloproteinase inhibitors (MMPIs) from marine natural products: the current situation and future prospects,”
  22. (2009). Matrix metalloproteinase-1 inhibitory activity of kaempferia pandurata roxb,”
  23. (2000). Matrix metalloproteinases and tissue inhibitors of metalloproteinases in synovial fluids from patients with rheumatoid arthritis or osteoarthritis,”
  24. (2000). Matrix metalloproteinases: multifunctional contributors to tumor progression,”
  25. (2003). Membrane type I matrix metalloproteinase usurps tumor growth control imposed by the three-dimensional extracellular matrix,”
  26. (2010). Non-apoptotic Fas signaling regulates invasiveness of glioma cells and modulates MMP-2 activity via NFκBTIMP-2 pathway,”
  27. (1993). Rapid proteolysis of IκB-α is necessary for activation of transcription factor NF-κb,”
  28. (2008). S.Das,A.Banerji,E.Frei,andA.Chatterjee,“Rapidexpression and activation of MMP-2 and MMP-9 upon exposure of h u m a nb r e a s tc a n c e rc e l l s(
  29. (2004). S.K.Moon,H.M.Kim,andC.H.Kim,“PTENinducesG1cell cycle arrest and inhibits MMP-9 expression via the regulation of NF-κB and AP-1 in vascular smooth muscle cells,”
  30. (2005). Suppression of tumor cell invasiveness by hydrolyzable tannins (plant polyphenols) via the inhibition of matrix metalloproteinase-2/-9 activity,”
  31. (2002). Sustained production of H2O2 activates pro-matrix metalloproteinase-2 through receptor tyrosine kinases/phosphatidylinositol 3-kinase/NF-κBp a t h w a y ,
  32. (2008). Tanshinone IIA from Salvia miltiorrhiza BUNGE inhibits human aortic smooth muscle cell migration and MMP-9 activity through AKT signaling pathway,”
  33. (2010). The ethyl alcohol extract of Hizikia fusiforme inhibits matrix metalloproteinase activity and regulates tight junction related protein expression in Hep3B human hepatocarcinoma cells,”
  34. The role of matrix metalloproteinases in tumor angiogenesis and tumor metastasis,” PathologyandOncologyResearch,vol.7,no.1,pp.14–23,2001.
  35. (2010). Vaccinium myrtillus (bilberry) extracts reduce angiogenesis in vitro and in vivo,”