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Electron Cryotomography of Bacterial Cells

By Songye Chen, Alasdair McDowall, Megan J. Dobro, Ariane Briegel, Mark Ladinsky, Jian Shi, Elitza I. Tocheva, Morgan Beeby, Martin Pilhofer, H. Jane Ding, Zhuo Li, Lu Gan, Dylan M. Morris and Grant J. Jensen


While much is already known about the basic metabolism of bacterial cells, many fundamental questions are still surprisingly unanswered, including for instance how they generate and maintain specific cell shapes, establish polarity, segregate their genomes, and divide. In order to understand these phenomena, imaging technologies are needed that bridge the resolution gap between fluorescence light microscopy and higher-resolution methods such as X-ray crystallography and NMR spectroscopy

Topics: Cellular Biology
Publisher: MyJove Corporation
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Provided by: PubMed Central

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