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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

By Charmaine Y. Pietersen, Maribel P. Lim and Tsung-Ung W. Woo

Abstract

We proposed to investigate the gray matter reduction in the superior temporal gyrus seen in schizophrenia patients, by interrogating gene expression profiles of pyramidal neurons in layer III. It is well known that the cerebral cortex is an exceptionally heterogeneous structure comprising diverse regions, layers and cell types, each of which is characterized by distinct cellular and molecular compositions and therefore differential gene expression profiles. To circumvent the confounding effects of tissue heterogeneity, we used laser-capture microdissection (LCM) in order to isolate our specific cell-type i.e pyramidal neurons

Topics: Neuroscience
Publisher: MyJove Corporation
OAI identifier: oai:pubmedcentral.nih.gov:3149916
Provided by: PubMed Central
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