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The Constitutive Expression of Type X Collagen in Mesenchymal Stem Cells from Osteoarthritis Patients Is Reproduced in a Rabbit Model of Osteoarthritis

By Fackson Mwale, Sonia Rampersad, Hélène Richard, Yao Guoying, Sora Al Rowas, Padma Madiraju, John Antoniou and Sheila Laverty

Abstract

The expression of type X collagen (COL X), a late-stage chondrocyte hypertrophy marker in human mesenchymal stem cells (MSCs) from osteoarthritis (OA) patients poses a major setback to current cartilage and intervertebral disc tissue engineering efforts. However, it is not yet clear whether COL X is expressed by all human bone marrow stem cells or if it is related to age, gender, site, disease status, or drug therapy. In the current study, we report that COL X expression is upregulated in MSCs from rabbits in a surgical instability model of OA (anterior cruciate ligament transection (ACLT)) when compared to control rabbit MSCs. Thus COL X expression in OA is a common phenomenon that is due to the disease process itself and not to other environmental factors. It is, therefore, critical to understand MSC phenotype in OA patients, as these cells are essential clinically for biological repair of cartilage lesions using autologous stem cells

Topics: Research Article
Publisher: SAGE-Hindawi Access to Research
OAI identifier: oai:pubmedcentral.nih.gov:3144696
Provided by: PubMed Central

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Citations

  1. (1998). A .M .M a c k a y ,S .C .B e c k ,J .M .M u r p h y ,F .P .B a r r y ,C .O . Chichester,andM.F.Pittenger,“Chondrogenicdifferentiation of cultured human mesenchymal stem cells from marrow,”
  2. (1998). a k i t a n i ,T .G o t o ,R .G .Y o u n g ,J .M .M a n s o u r
  3. (2001). Age- and gender-related changes in the cellularity of human bone marrow and the prevalence of osteoblastic progenitors,”
  4. Analysis of the type of collagen present in osteoarthritic human cartilage,”
  5. Application of chitosanbased polysaccharide biomaterials in cartilage tissue engineering: a review,”
  6. (1999). Articular cartilage repair: are the intrinsic biological constraints undermining this process insuperable?”
  7. (2000). Articular cartilage repair: problems and perspectives,”
  8. (1997). Cartilage in health and disease,” in Arthritis and
  9. (2001). Chondrocytic differentiation of mesenchymal stem cells sequentially exposed to transforming growth factor-β1 in monolayer and insulin-like growth factorI in a three-dimensional matrix,”
  10. (2003). Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads,”
  11. (2004). Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells in pellet cultural system,”
  12. (2001). Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components,”
  13. (2004). Chondrogenic differentiation of murine embryonic stem cells: effects of culture conditions and dexamethasone,”
  14. (2005). Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation,”
  15. (2003). Distinct phases of coordinated early and late gene expression in growth plate chondrocytes in relationship to cell proliferation, matrix assembly,remodeling,andcelldifferentiation,”JournalofBone and
  16. (2005). Effe c to fo r a l glucosamine on cartilage degradation in a rabbit model of osteoarthritis,”
  17. (2001). Effect of different growth factors on the chondrogenic potential of human bone marrow stromal cells,”
  18. (2000). Effect of hyaluronan on chondrocyte apoptosis and nitric oxide production in experimentally induced osteoarthritis,”
  19. (2010). Effect of parathyroid hormone on type X and type II collagen expression in mesenchymal stem cells from osteoarthritic patients,”
  20. (2000). Effect of transforming growth factor β1 on chondrogenicdifferentiationofculturedequinemesenchymalstem cells,”
  21. (2004). Effects of dehydroepiandrosterone on articular cartilage during the development of osteoarthritis,”
  22. (2000). Endochondral bone formation and development in the axial and appendicular skeleton,”
  23. (1997). Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage,”
  24. (1999). Enhanced repair of extensive articular defects by insulinlike growth factor-I-laden fibrin composites,”
  25. (1988). Epidemiology of hip and knee osteoarthritis,”
  26. (2008). Evaluation of autologous chondrocyte transplantation via a collagen membrane in equine articular defects: results at 12 and 18 months,” Osteoarthritis and Cartilage,
  27. (2000). F.Mwale,C.Billinghurst,W.Wuetal.,“Selectiveassemblyand remodelling of collagens II and IX associated with expression of the chondrocyte hypertrophic phenotype,”
  28. (1986). G i b s o n ,C .H .B e a r m a n ,a n dM .H .F l i n t ,“ T h ei m m u -noperoxidase localization of type X collagen in chick cartilage andlung,”CollagenandRelatedResearch,vol.6,no.2,pp.163– 184,
  29. (1989). Immunoelectron microscopic studies of type X collagen in endochondral ossification,”
  30. (1990). Immunoelectron microscopy of type X collagen: supramolecular forms within embryonic chick cartilage,”
  31. (1985). Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues,”
  32. (1976). Immunohistological study on collagen in cartilagebone metamorphosis and degenerative osteoarthrosis,”
  33. (1998). In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells,”
  34. (1994). Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay,”
  35. (1992). K .v o nd e rM a r k ,T .K i r s c h ,A .N e r l i c he ta l . ,“ T y p eXc o l l a g e n synthesis in human osteoarthritic cartilage: indication of chondrocyte hypertrophy,”
  36. (1973). K e m p s o n ,H .M u i r ,C .P o l l a r d ,a n dM .T u k e ,“ T h e tensile properties of the cartilage of human femoral condyles related to the content of collagen and glycosaminoglycans,”
  37. (2006). Limitations of using aggrecan and type X collagen as markers of chondrogenesis in mesenchymal stem cell differentiation,”
  38. (2002). M u r p h y ,K .D i x o n ,S .B e c k ,D .F a b i a n ,A .F e l d m a n ,a n d
  39. (2010). Novel insights into the mechanism of decreased expression of type X collagen in human mesenchymal stem cells from patients with osteoarthritis cultured on nitrogen-rich plasma polymers: implication of cyclooxygenase-1,”
  40. (2000). o n o d a ,F .L .H a r w o o d ,M .E .A m i e l ,H .M o r i y a ,a n dD . Amiel,“Theeffectsofhyaluronanonthemeniscusintheanterior cruciate ligament-deficient knee,”
  41. (1996). o s h i o k a ,R .D .C o u t t s ,D .A m i e l ,a n dS .A .H a c k e r , “Characterization of a model of osteoarthritis in the rabbit knee,” Osteoarthritis and Cartilage,
  42. (1996). O.Jacenko,S.Ito,andB.R.Olsen,“Skeletalandhematopoietic defects in mice transgenic for collagen
  43. (2009). Osteoarthritis treated with mesenchymal stem cells on Hyaluronan-based scaffoldinrabbit,”TissueEngineeringC,vol.15,no.4,pp.647– 658,
  44. Ouellet et al., “Calcification in human intervertebral disc degeneration and scoliosis,” Journal of Orthopaedic Research.
  45. (2005). Selective inhibition of type X collagen expression in human mesenchymal stem cell differentiation on polymer substrates surface-modified by glow discharge plasma,”
  46. Stem cells, nitrogenrich plasma-polymerized culture surfaces and type X collagen suppression,” Tissue Engineering A. In press.
  47. (2006). Suppression of genes related to hypertrophy and osteogenesis in committed human mesenchymal stem cells cultured on novel nitrogen-rich plasma polymer coatings,”
  48. (2002). The assembly and remodeling of the extracellular matrix in the growth plate in relationship to mineral deposition and cellular hypertrophy: an in situ study of collagens II and IX and proteoglycan,”
  49. (2006). The effect of glow discharge plasma surface modification of polymers on the osteogenic differentiation of committed human mesenchymal stem cells,”
  50. (2010). The OARSI histopathology initiative— recommendations for histological assessments of osteoarthritis
  51. (1983). The untreated anterior cruciate ligament rupture,”
  52. (2003). Transforming growth factorβ-mediated chondrogenesis of human mesenchymal progenitor cells involves N-cadherin and mitogen-activated protein Kinase and wnt signaling cross-talk,”
  53. (1994). Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation,” The New England
  54. (1998). Use of mesenchymal stem cells in a collagen matrix for Achilles tendon repair,”
  55. (2009). Use of routine clinical multimodality imaging in a rabbit model of osteoarthritis—part II: bone mineral density assessment,”