Article thumbnail

Evodia rutaecarpa and Three Major Alkaloids Abrogate Influenza A Virus (H1N1)-Induced Chemokines Production and Cell Migration

By Wen-Fei Chiou, Han-Chieh Ko and Bai-Luh Wei

Abstract

Evodia rutaecarpa is commonly used as an anti-inflammatory herbal remedy in traditional Chinese medicine. In this study, the ethanol extract of E. rutaecarpa (ER) and three major quinazoline alkaloids dehydroevodiamine (DeHE), evodiamine (Evo) and rutaecarpine (Rut), isolated from ER were employed to study their inhibitory effects against influenza A virus (H1N1)-induced chemokines production in A549 lung epithelial cells as well as on chemokines-evoked cell recruitment in HL-60-differentiated macrophages. The results showed that ER was a potent inhibitor of RANTES secretion by H1N1-inoculated A549 cells (IC50: 1.9 ± 0.4 μg ml−1). Three alkaloids, although to differing extents, all concentration dependent, inhibited H1N1-induced RANTES production with Evo consistently being the most potent among these active components. ER also moderately and significantly inhibited H1N1-stimulated MCP-1 production in A549 cells. This was mimicked by Evo and Rut, but not DeHE. In the macrophage recruitment assay, both RANTES and MCP-1 markedly evoked cell migration and this phenomenon was significantly suppressed by ER. Evo and Rut, but not DeHE, also had the ability to inhibit cell migration toward RANTES and MCP-1, respectively. In summary, three major alkaloids displayed different potentials for inhibiting chemokines secretion and subsequently cell migration, which could partially explain the activity of ER. As an effective agent to suppress H1N1-induced chemokines production and block chemokine-attracted leukocytes recruitment, E. rutaecarpa and its active components may be useful in influenza virus infection-related inflammatory disorders

Topics: Original Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:3139406
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (2002). A cathelicidin family of human antibacterial peptide
  2. (2004). Andrographolide acts through inhibition of ERK1/2 and Akt phosphorylation to suppress chemotactic migration,”
  3. (2007). Anti-inflammatory effects and mechanisms of the ethanol extract of Evodia rutaecarpa and its bioactive components on neutrophils and microglial cells,”
  4. (2002). Anti-invasive and metastatic activities of evodiamine,”
  5. (1998). Antinociceptive and anti-inflammatory activities of limonin isolated from the fruits of Evodia rutaecarpa var.
  6. (2006). ar osh,J .W .Galvin,S.L.N ay ,A.V .P e˜ na,M.T.
  7. (1999). B o n v i l l e ,H .F .R o s e n b e r g ,a n dJ .B .D o m a c h o w s k e , “Macrophage inflammatory protein-1α and RANTES are present in nasal secretions during ongoing upper respiratory tract infection,” Pediatric Allergy and Immunology,
  8. (1987). C h a n ga n dP
  9. (2002). C h i o u ,H .C .K o ,C .F .C h e n ,a n dC .J .C h o u ,“ Evodia rutaecarpa protects against circulation failure and organ dysfunction in endotoxaemic rats through modulating nitric oxide release,”
  10. (2006). C h o i
  11. (2004). Characterization of the MEK5-ERK5 module in human neutrophils and its relationship to ERK1/ERK2 in the chemotactic response,” J o u r n a lo fB i o l o g i c a lC h e m i s t r y ,
  12. (2007). Cryptotanshinone inhibits chemotactic migration in macrophages through negative regulation of the PI3K signaling pathway,”
  13. (2008). Cytokine storm in avian influenza,”
  14. (2002). Determination of dehydroevodiamine, evodiamine, rutaecarpine and synephrine in Evodia genus plants from Taiwan and mainland China,”
  15. (2005). Dual regulatory effect of plant extracts of Forsythia suspense on RANTES and MCP1 secretion in influenza A virus-infected human bronchial epithelial cells,”
  16. (1992). Evodia rutaecarpa (Juss)Benth,”
  17. (2009). Evodiamine and rutaecarpine inhibit migration by LIGHT via suppression of NADPH oxidase activation,”
  18. (2009). Evodiamine represses hypoxia-induced inflammatory proteins expression and hypoxia-inducible factor 1α accumulation
  19. (1997). Expression of cytokines on human bronchial epithelial cells induced by influenza virus
  20. (1998). Expression of RANTES by normal airway epithelial cells after influenza virus A infection,”
  21. (2008). H u a n g ,S .H .L o k e ,C .C .H s u ,a n dW .F .C h i o u , “(+)-Vitisin A inhibits influenza a virus-induced RANTES production in 549 alveolar epithelial cells through interfering with Akt andSTAT1 phosphorylation,”
  22. (2000). Influenza A and Sendai viruses induce differential chemokine gene expression and transcription factor activation in human macrophages,”
  23. (2004). Inhibition by evodiamine of hepatocyte growth factor-induced invasion and migration of tumor cells,”
  24. (2004). Inhibition of RANTES expression by indirubin in influenza virus-infected human bronchial epithelial cells,”
  25. (1997). Inhibitory effect of dehydroevodiamine and evodiamine on nitric oxide production
  26. Inhibitory effects of evodiamine on in vitro invasion and experimental lung metastasis of murine colon cancer cells,”
  27. (2000). Loetscher,“Chemokinesin inflammation and immunity,”
  28. (2003). Quantitatively evaluation of bioactive components of Evodia rutaecarpa (Tetradium ruticarpum) in different harvesting times,”
  29. (1998). Respiratory syncytial virus-induced RANTES production from humanbronchial epithelial cells is dependent on nuclear factor-oB nuclear binding and is inhibited by adenovirusmediated expression of inhibitor of oB±,”
  30. (1995). The effect of Evodia rutaecarpa extract on cytokine secretion by human mononuclear cells in vitro,”