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Evaluation of In Vitro Uterotonic Activities of Fruit Extracts of Ficus asperifolia in Rats

By Pierre Watcho, Esther Ngadjui, Pepin Alango Nkeng-Efouet, Telesphore Benoît Nguelefack and Albert Kamanyi

Abstract

The aim of the present study was to determine the uterotonic activities of Ficus asperifolia and investigate its mechanism. The effects of aqueous and methanol extracts of the dried fruits of F. asperifolia (0.05–1.60 mg mL−1) were evaluated on estrogenized isolated rat uterus in the presence and absence of atropine (1.73–55.27 nM), pyrilamine maleate (1.25 × 10−3 to 40 × 10−3 M), indomethacin (0.06 × 10−5 to 2.00 × 10−5 M) or hexamethonium (0.66 × 10−4 to 21.43 × 10−4 M). Aqueous (EC50, 0.36 mg mL−1) and methanol (EC50, 0.22 mg mL−1) extracts as well as oxytocin (EC50, 0.02 nM), acetylcholine (EC50, 7.87 nM) and histamine (EC50, 0.76 nM) evoked concentration-dependent contractions of the uterus. Atropine, pyrilamine maleate and indomethacin concentration dependently blocked the response of the uterus to acetylcholine (IC50, 4.82 nM), histamine (IC50, 2.49 nM) and oxytocin (IC50, 0.07 nM), respectively, and to aqueous extract. Hexamethonium produced graded decreases in oxytocin-induced uterine contractions (IC50, 0.37 μM), but did not prevent the contractile effects of the aqueous extract (IC50, 9.88 μM). These results suggest that F. asperifolia-induced uterotonic effect is related to the release of prostaglandins and contraction of the myometrial cells through muscarinic, oxytocic and H1 histamine receptors. These data further give added value to the ethnic use of F. asperifolia for its abortificient and contraceptive properties

Topics: Original Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:3137561
Provided by: PubMed Central

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Citations

  1. (2006). A d a m sJ r .a n dC .G a r c i a ,“ W o m e n ’ sh e a l t ha m o n gt h e Chumash,” Evidence-Based Complementary and Alternative Medicine,
  2. (2008). a n g ,a n dZ .H . Zhao, “Active pharmaceutical ingredients and mechanisms underlying phasic myometrial contractions stimulated with the saponin extract from Paris polyphylla Sm. var. yunnanensis used for abnormal uterine bleeding,”
  3. (2007). a n g u l y ,M .K r . B o r t h a k u r ,N .D e v i ,a n dR .M a h a n t a , “Antifertility activity of the methanolic leaf extract of Cissampelos pareira in female albino mice,”
  4. (1993). a r t i n e z - M i r ,J .H e r r e r o ,L .E s t a n ,F .J .M o r a l e s - O l i v a s ,a n d E. Rubio, “Effect of histamineon the longitudinal and circular muscle of the oestrogen dominated rat uterus,”
  5. (2003). Cellular signaling mechanisms for muscarinic acetylcholine receptors,”
  6. (1993). Characterization of muscarinic receptors in guinea-pig uterus,”
  7. (2007). Contraceptive and hormonal properties of the stem bark of Dysoxylum binectariferum in rat and docking analysis of rohitukine, the alkaloid isolated from active chloroform soluble fraction,”
  8. (2002). Estrogenicandcholinergicproperties ofthemethanolextract of Ruellia praetermissa Sceinf. ex. Lindau (Acanthaceae) in female rats,”
  9. (1994). International union of pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes,”
  10. (1970). La Chimie des St´ ero¨ ıdes, Gauthier-Villars,
  11. (2009). M.T.YakubuandB.B.Bukoye,“Abortifacientpotentialsofthe aqueous extract of Bambusa vulgaris leaves in pregnant
  12. (1993). Oxytocic receptor subtypes in the pregnant rat myometrium and deciduas: pharmacologicaldifferentiations,”
  13. (1999). Pharmacognosy—Phytochemistry of Medicinal Plants,
  14. (1995). Pharmacologic stimulation of uterine contraction,”
  15. (1989). Pharmacological characterization of the muscarinic receptors mediating phosphoinositide hydrolysis in rat myometrium,”
  16. (2000). Pharmacological effects of the aqueous extract of Neorautanenia mitis in rodents,”
  17. (1996). Plantes M´ edicinales et M´ edecine Traditionnelle d’Afrique, Edition Karthala,
  18. (2004). Population Reference Bureau,
  19. (2008). Post-coital antifertility activity of Hibiscus rosa-sinensis Linn.
  20. (2007). S r i v a s t a v a ,G .K e s h r i ,B .B h a r g a v a n ,C .S i n g h ,a n dM
  21. (1995). Stimulating action of Carthamus tinctorius L., Angelica sinensis (Oliv.) Diels and Leonurus sibiricus L. on the uterus,” Zhongguo Zhong Yao Za Zhi,
  22. (1998). Stimulation of autonomic cholinoceptors in the rat uterus by a crude extract from eleophorbia drupifera leaves,”
  23. (1982). Techniques of Flavonoid Identification,A c a -demic Press,
  24. (2000). The effectsofherbal oxytocics on the isolated “stripped” myometrium model,”
  25. (1998). Uterotonic properties of the methanol extract of Monechma ciliatum,”
  26. (1999). V e a l e ,I .H a v l i k ,D .W .O l i v e r ,a n dT .G .D e k k e r , “Pharmacological effects of Agapanthus africanus on the isolated rat uterus,”
  27. (2008). W i l l e t s ,A .H .T a y l o r ,H .S h a w ,J .C .K o n j e ,a n dR