Article thumbnail

Antioxidant and Anti-Cancer Potentials of Rheum emodi Rhizome Extracts

By Venkatadri Rajkumar, Gunjan Guha and Rangasamy Ashok Kumar

Abstract

The objective of this study was to determine antioxidant and cytotoxic efficacies of methanolic and aqueous extracts of Rheum emodi Wall. ex Meissn. rhizome. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, inhibitory effect on lipid peroxidation and Fe3+ reducing antioxidant property have been used to investigate antioxidant properties of the extracts. Cytotoxicity of the extracts was tested on MDA-MB-435S and Hep3B cell lines. Both extracts displayed extensive cytotoxicity to the tested cell lines. The extracts were studied for their ability to protect pBR322 DNA from damage by UV induced photolysis of H2O2. The aqueous extract, though inferior to methanolic extract in its antioxidant potential exhibited efficiency in DNA protection, while the methanolic extract failed to protect the DNA. The amount of total polyphenolics in the extracts was measured by spectrophotometric method. The methanolic extract contained higher polyphenolic contents than aqueous extract. Significant positive correlations were observed (P < .05) between results of phenolic content estimation and that of antioxidant assays. Hence, high-performance liquid chromatography analysis was performed to identify few major phenolic compounds that might be responsible for these therapeutic properties. These results indicate that rhizome of R. emodi possesses antioxidant and cytotoxic activities and therefore have therapeutic potential

Topics: Original Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:3137233
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (1976). A g a r w a l ,R .P .R a s t o g i ,a n dO .P .S r i v a s t a v a ,“ In vitro toxicity of constituents of Rumex maritimus Linn. to ringworm fungi,”
  2. (1987). Antifungal and other compounds isolated fromtherootsofNew Zealand flax plants (the genus
  3. (1958). Antioxidant determinations by the use of a stable free radical,”
  4. (1976). Approaches to prevention of epithelial cancer during the preneoplastic period,”
  5. (1988). Biochemical study of Chinese rhubarb. XXII. Inhibitory effect of anthraquinone derivatives on sodium-potassium-ATPase of the rabbit renal medulla and their diuretic action,”
  6. (1989). Biochemical study of Chinese rhucarb. XXIX. Inhibitory effects of anthraquinone derivatives on P388 leukemia in mice,”
  7. (1987). C y o n g ,T .M a t s u m o t o ,K .A r a k a w a ,H .K i y o h a r a
  8. (1985). Determination of the laxative compounds in rhubarb by high performance liquid chromatography,”
  9. (2000). Di ll a r da n dJ .B ru c eG e rm a n ,“ P h yt oc h e m i c a l s :n u trac e u -ticals and human health,”
  10. F u z e l l i e r ,F .M o r t e r ,a n dP .L e c t a r d ,“ A n t i f u n g a la c t i v i t y of Cassia alata
  11. (1992). Free radicals, antioxidants and human disease: where are we now?”Evidence-Based
  12. (2003). Germinability, productivity and economic viability of Rheum emodi Wall. ex Meissn. cultivated at lower altitude,” Current Science,v o l .8 4 ,n o .2 ,p p .
  13. (2001). Hypoxia in oxidative stress in breast cancer: oxidative stress—its effects on the growth, metastatic potential and response to therapy of breast cancer,”
  14. (2001). Indian medicinal plants as antiradicals and DNA cleavage protectors,”
  15. (1995). L¨ o l i g e r ,a n dO .I .A r u o m a , “The characterization of antioxidants,”
  16. (1991). Lipid peroxidation—mechanism and biological relevance,” in Free Radicals and Food Additives,O .I .A r u o m a
  17. (1989). New soluble-formazan assay for HIV1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity,”
  18. (1981). Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system,”
  19. (2003). Simultaneous determination of all polyphenols in vegetables, fruits, and teas,”
  20. (1986). Studies on antioxidant; their carcinogenic and modifying effects on chemical carcinogensis,”
  21. (1990). Stuffn e s sa n dJ .M .P e z z u t o ,“ A s s a y sr e l a t e dt oc a n c e r drug discovery,” in Methods in Plant Biochemistry: Assays for Bioactivity,
  22. (2002). The comet assay with 8 mouse organs: results with 39 currently used food additives,”
  23. The ferric reducing ability of plasma(FRAP)asameasureof“antioxidantpower“:theFRAP assay,”AnalyticalBiochemistry,vol.239,no.1,pp.70–76,1996.
  24. (1988). The nature of oxidant and antioxidant systems in the inhibition of mutation and cancer,”
  25. (2002). u ,S .H a l e y ,J .P e r r e t ,M .H a r r i s ,J .W i l s o n ,a n dM .Q i a n , “Free radical scavenging properties of wheat extracts,”
  26. (2007). u r v e s w a r a n ,Y . - Z .C a i ,H .C o r k e ,a n dM .S u n ,“ S y s t e m a t i c evaluation of natural phenolic antioxidants from 133 Indian medicinal plants,”