Article thumbnail

Quantitative Detection of µ Opioid Receptor: Western Blot Analyses Using µ Opioid Receptor Knockout Mice

By Shinya Kasai, Hideko Yamamoto, Etsuko Kamegaya, George R Uhl, Ichiro Sora, Masahiko Watanabe and Kazutaka Ikeda


Increasing evidence suggests that µ opioid receptor (MOP) expression is altered during the development of and withdrawal from substance dependence. Although anti-MOP antibodies have been hypothesized to be useful for estimating MOP expression levels, inconsistent MOP molecular weights (MWs) have been reported in studies using anti-MOP antibodies. In the present study, we generated a new anti-MOP antibody (N38) against the 1-38 amino acid sequence of the mouse MOP N-terminus and conducted Western blot analysis with wildtype and MOP knockout brain lysates to determine the MWs of intrinsic MOP. The N38 antibody detected migrating bands with relative MWs of 60-67 kDa in the plasma membrane fraction isolated from wildtype brain, but not from the MOP knockout brain. These migrating bands exhibited semi-linear density in the range of 3-30 µg membrane proteins/lane. The N38 antibody may be useful for quantitatively detecting MOP

Topics: Article
Publisher: Bentham Science Publishers Ltd
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (1995). Antibodies to the cloned μ-opioid receptor detect various molecular weight forms in areas of mouse brain.
  2. (2001). C-terminal splice variants of the mouse μ-opioid receptor differ in morphine-induced internalization and receptor resensitization.
  3. (2005). Chronic ethanol modulates delta and mu-opioid receptor expression in rat CNS: immunohistochemical analysis with quantitiative confocal microscopy.
  4. (1999). Chronic prenatal cocaine treatment down-regulates μ-opioid receptor mRNA expression in the brain of fetal Rhesus Macaque.
  5. (2005). Correlation of stable elevations in striatal -opioid receptor availability in detoxified alcoholic patients with alcohol craving: a positron emission tomography study using carbon 11-labeled carfentanil.
  6. (1995). Distribution and targeting of a μ-opioid receptor (MOR1) in brain and spinal
  7. (1996). Effect of chronic cocaine treatment on μ- and -opioid receptor mRNA levels in dopaminergically innervated brain regions.
  8. (2002). Exploring the opioid system by gene knockout.
  9. (2005). Immediate withdrawal from chronic “binge” cocaine administration increases μ-opioid receptor mRNA levels in rat frontal cortex. Brain Res.
  10. (2000). Immunoprecipitation of high-affinity, guanine nucleotide-sensitive, solubilized μ-opioid receptors from rat brain: coimmunoprecipitation
  11. (2004). Mu-opioid receptor binding measured by [ 11C]carfentanil positron emission tomography is related to craving and mood in alcohol dependence.
  12. (1997). Opiate receptor knockout mice define μ receptor roles in endogenous nociceptive responses and morphine-induced analgesia.
  13. (2005). Pharmacogenetics and human molecular genetics of opiate and cocaine addictions and their treatments.
  14. (2005). Ultralow-dose naloxone suppresses opioid tolerance, dependence and associated changes in mu opioid receptor-G protein coupling and G signaling. Neuroscience,